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Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx

Báo cáo y học:

Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx

... aerial part of A. paniculata is commonly used in Chinese medicine.According to Chinese medicine theory, A. paniculata 'cools' and relieves internal heat, inflammation and pain and is used ... effects in treating acute upper respira-tory tract infections and relieving the inflammatorysymptoms of sinusitis [85].The migratory response of leukocytes to chemokinesforms the first line of ... inhibits HIV-induced cellcycle dysregulation, leading to a rise in CD4+ lymphocytelevels in HIV-1 infected individuals [80].Andrographolide, neoandrographolide and 14-deoxy-11,12-didehydroandrographolide...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

... proteins[8,23] and to identify in vitro the most reactive internalcysteine (Cys207) of Sorghum NADP-MDH [12]. Thecytosolic Trx h of Chlamydomonas has only two cysteines in its primary sequence, ... 2Cys-Prx subgroup, and 1Cys-Prx subgroup containtwo and one conserved cysteines, respectively. 2Cys-Prxproteins are reduced by the AhpF protein in bacteria, and by the thioredoxin/thioredoxin ... dif®culty,thioredoxin-de®cient yeast or E. coli mutant strains havebeen used to express a single-cysteine m utant thioredoxinallowing the i solation of thioredoxin targets in y east [8] and Fig....
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

... FEBS 2002mass ranging from 95 to over 100 kDa in polyacrylamidegels. Antibody staining of sections from bovine prelactating and lactating mammary gland using monoclonal and polyclonal antibodies ... 2-propanol were collected and freeze-dried. Standard procedures were employed analysing pro-tein samples by SDS/PAGE using 18% polyacrylamidegels, and for the staining of proteins using CoomassieBrilliant ... surface in epithelial cells of the mammarygland. Likewise, the deduced amino-acid sequences of bothbovine and human MUC15 resemble the mucins in havingserine, threonine and proline as the predominant...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

... 72.7Ofloxacin00Cefixime00Polymyxin B0 36.4Cefotaxime0 18.2Sparfloxacin00Cephalothin18.2 27.3Streptomycin0 36.4Chloramphenicol00Tetracycline18.2 36.4Ciprofloxacin00Tobramycin00Erythromycin100 0Trimethoprim/sulfamethoxazole00Gentamicin00Imipenem00Molecular ... followed by EtBr staining and photographed under UV illumination.RAPD fingerprintingTo increase the reproducibility of RAPD analysis, twokinds of 10-mer random primers (referred as CRA22 and CRA23) ... P010726-25, and O157-C-1-2), and 14 USAisolates; 4 strains of ATCC (A1, A2, A3, and A4), 6 strains of Cornell University (C1, C2, C3, C4, C5, and C6), and 4strains of Pennsylvania State University (P3,...
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Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt

... the clinical Isolation and identification of a canine coronavirus strain from giant pandas 263Fig. 3. Analysis of GPV based on gene sequencing. (A) Comparison of amino acid sequences of S gene ... indicates the cytoblast of an infected FCWF cell. The black arrow indicates the inclusion bodies of GPV in the cytoplasm of cells. Scale bars = 200 nm. 10 volumes of Hank’s solution containing 10% ... supernatant by negative staining. The arrows indicate the corona spikes of the GPV particles. (B) Detection of inclusion bodies of GPV in FCWF cells by ultrathin section. The white arrow indicates...
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Báo cáo y học: " Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan" pptx

... proteins. By in creasing the temperature of the heat treatment to 70°C, band X faded, whereasthe intensity of band Y increased. With further increases in temperature, band Y faded, whereas the intensity ... glycine, and 25 mM Tris.N-terminal amino acid sequence analysis and amino acidsequence homology searchAfter SDS-PAGE, proteins in the polyacrylamide gelswere electroblotted onto polyvinylidene ... than those infected by CvV-BW3)using the streptavidin-biotin system, indicating the pro-duction of hyaluronan by CvV-BW1 (Fig. 9).DNA polymerase gene phylogeny of CvV-BW1The DNA polymerase genes,...
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Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot

... lymphocytes) following chemical (cytokines,thrombin and endotoxin), physical (shear stress and hypoxia) [17] and apoptotic [18] stimuli. One of the firstdescribed roles for MPs was in the initiation ... from 50%, 70%, 95% and 100% for 10 minutes in each solution. The sample was finally dehydrated in pro-pylene oxide for 15 minutes. Finally, the sample wasembedded in epoxy resin (Epon 12). After ... efficacy of noveltherapies [31]. Sputum is a rich, non-invasive source of biomarkers of inflammation and infection, and has beenused extensively to assess inflammation in the CF airways(reviewed...
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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf

... University of Minnesota, Minneapolis, Minnesota, USA and 2Dept. of Genetics, Cell Biology and Development and the Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, ... efficiency was determined at the hypoxan-thine guanine phosphoribosyl transferase (HPRT) locus and at the adenine phosphoribosyltransferase locus byplating in medium containing 6-thioguanine (6-TG) ... 42-7cell lineD)E)NUCLEUSCYTOPLASM01x1052x1053x1054x105Kinetics of proviral DNA synthesis in wild-type and 30-2 cellsFigure 5Kinetics of proviral DNA synthesis in wild-type and 30-2...
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Báo cáo y học: "Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets" ppt

... as those for hypothetical proteins,expressed proteins and putative proteins, are not included in the target prediction because of the difficulty in identifyingtheir orthologs in the O. sativa ... miR-NAs bind to the protein-coding region of their mRNA targetwith nearly perfect sequence complementarity, and degradethe target mRNA in a way similar to RNA interference (RNAi)[29]. Analysis of ... Ikeda Y, Morgante M, Wang X, Zuo J, Hanafey MK, Gaaster-land T, Tingey SV, Chua NH: Monitoring genome-wide changes in gene expression in response to endogenous cytokininreveals targets in Arabidopsis...
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx

Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx

... 2002D14-SR. To verify our hypothesis, bovine cDNA encodingthe 38-kDa protein was cloned to iden tify the catalyticactivity of the expressed protein.Cloning of the cDNA encoding bovine sterolD14-reductaseBovine ... DISCUSSIONIsolation of sterol D14-reductaseDuring the preparation and delipidation of a bovine liver38-kDa protein exhibiting EPT activity [25], a protein co-migrating in SDS/PAGE was revealed by amino-acidsequence ... Incubation was carried out under N2for30 min at 37 °C with 0.24 mg of microsomal proteins and terminated by the addition of 1 mL of 20% KOH in 50%methanol, followed by additional 30 min incubation...
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