Báo cáo y học: "Liver sinusoidal endothelial cells represents an important blood clearance system in pigs" doc

... HepatologyOpen AccessResearchLiver sinusoidal endothelial cells represents an important blood clearance system in pigsGeir I Nedredal*1, Kjetil H Elvevold2, Lars M Ytrebø1, Randi Olsen3, ... centrifugation, and centrifugal elutriation. Thismethod yields both high purity and functionally intact pigliver sinusoidal cells that can be cultivated in monolayercultures. Notably, the yield of sinusoidal ... studies in rats and a few other mammalian species, including man, haveshown that the sinusoidal cells constitute an important part of liver function. In the pig, however,which is frequently used in...

Báo cáo y học: " Liver sinusoidal endothelial cell modulation upon resection and shear stress in vitro" doc

... Zhang G, Liu D: Hydrodynamics-based transfec-tion: simple and efficient method for introducing andexpressing transgenes in animals by intravenous injection ofDNA. Methods Enzymol 2002, 346:92-105.31. ... thereby.BackgroundFollowing partial hepatectomy (PHx) the remaining liveris transfused by normal blood volume, thereby exposingliver sinusoidal endothelial cells (LECs) to excess hemo-dynamic ... 3a). Ten minutes afterhepatectomy, many blood platelets adhered to the endothelial lining. In addition, the endothelial liningbecame disrupted as represented by the occurrence of gapsand microvilli,...

Báo cáo y học: "Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation" doc

... morphology (Figure 7h and inset). In contrast,CD163+ cells were fewer in number (Figure 7f) and most werelocated in the synovial lining (type A synoviocytes), with onlyscattered cells in the subintimal ... first 6 days, PMN cells and typical inflammatory mono-cytes did not increase until days 9 to 12 and days 12 to 14,respectively. The delayed appearance of MHC II- cells (mainlyPMN cells) , which ... Alan Bishop and Sandy Macintyre (Detmold Family Trust Cell Imaging Centre, Hanson Research Institute, Adelaide, Australia) for their advice and assistance in flow cytometry and cell sorting.References1....

Báo cáo y học: "γ The role of the complement and the FcγR system in the pathogenesis of arthritis" pdf

... for injury to skin and peritoneum [53].Another elegant study in an IC-induced inflammation modelargued for a codominant role of inflammatory pathwaysmediated by FcγRI/III and C5aR [54]. In ... cells, macrophages, granulocytes andparticular neutrophils into the synovial lining and fluid and theperiarticular spaces. These infiltrating cells produce abundantcytokines, dominated mainly ... or bythe direct engagement and activation of FcγR-bearinginflammatory cells. Complement and FcγRs act in concert in many inflammatory responses, with complement bothattracting and activating...

Báo cáo y học: "RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast" docx

... diamide. In both cases, changes in mRNA levels were well-correlated with changes in PolII occupancy, and in general PolII changes typicallyexplain approximately 50% of the variance in mRNAchanges. ... changesand changes in mRNA abundance, but there was signifi-cant variation as well - changes in PolII abundance typi-cally accounted for approximately 50% of variance in mRNA abundance in ... microarray hybridizations, MY assisted withdata analysis, N F participated i n experiment planning and manuscript preparation,SB participated in experiment plann ing and manuscript preparation, an...

Báo cáo y học: " Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis" doc

... mapping pro-cesses can be difficult if the mutant phenotype is subtleand if assaying the phenotype is labor intensive.The circadian clock is an endogenous 24-h timerfound in most eukaryotes and ... photosynthetic bacteria. In plants, the clock plays a key role driving rhythms in physiology, biochemistry and metabolism [7]. In Arabi-dopsis, our current model of the clock is a series ofinter-locking ... affectequally the clock output in darkness (as manifested by an early phase) and light, suggesting it has a light-inde-pendent effect, and its primary defect may thereforenot be in the light signaling...

Báo cáo y học: "Does carbon monoxide treatment alter cytokine levels after endotoxin infusion in pigs? A randomized controlled study" pptx

... together with our findings is that CO may have an anti-inflammatory effect in mice but not in humans or pigs.The cytokine levels following endotoxin infusion in ourstudy were high, and individual ... that administration of CO hadany effect on cytokine release during endotoxin-inducedinflammatory response. Pro-inflammatory cytokines(TNF-alpha, IL-6 and IL-1beta) were neither attenuated in CO-treated ... found in a control animal thatTable 1: Circulatory and respiratory data from pigs during endotoxin infusion.-60 (min) 0 (min) 60 (min) 120 (min) 210 (min) 300 (min)group mean ± sem mean ± sem...

Báo cáo y học: "The effect of tiotropium therapy on markers of elastin degradation in COPD" doc

... Desmosine and Isodesmosine (D/I) are cross-linking amino acids which are presentonly in mature elastin. Changes in their concentration in body fluids indicate changes in elastindegradation and can ... demon-strated in lung parenchyma. Recently, techniques fordetecting and quantifying elastin degradation in body flu-ids have advanced in specificity, sensitivity and accuracyby the use of mass ... dem-onstrated to inhibit allergin-induced airway remodeling in a Guinea pig model of ongoing asthma[10]. Thus,endogenous acetylcholine may be an important mediator in airway smooth muscle remodeling in asthma,...

Báo cáo y học: "RNA interference for CFTR attenuates lung fluid absorption at birth in rats" doc

... linked to preterm birth,inherited genetic diseases, and inflammation and mayincrease the risk of hypoxic injury to vital organs in new-born infants; such injuries representing ~4% of infantfatalities ... siRNA-generating pDNAs invoked an inter-feron and/or cytokine response. Since both ENaC andCFTR can be affected by cytokines and interferons [35,36],this could potentially explain the downregulation ... in lungliquid clearance and electrolyte balance in newborn mice.Insights into perinatal adaptation and pseudohypoaldos-teronism. J Clin Invest 1998, 102:1634-1640.21. Snouwaert JN, Brigman...

Báo cáo y học: " Neutrophil cannibalism – a back up when the macrophage clearance system is insufficient" pot

... clearance of pathogensduring infection by killing and phagocyting pathogens. In the present study, using a model of LPS-induced lunginflammation we propose yet another mechanism bywhich they ... the clearance and resolution of an inflammation by removingpro-inflammatory cell debris from the tissue, thereby act-ing as a back up system stepping in when the macrophage system is exhausted. ... resolution of inflammation:by phagocytosing apoptotic cells and/or cell remnants.Neutrophils are classified as professional phagocytes, andare important in resolution and clearance of pathogens[1,2]....

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