... 5¢-CGGGATCCTCACTCCAAGGACACGGCA-3¢;DR5forward,5¢-CGGAATTCTGCAAGTCTTTACTGTGGAA-3¢; DR5 reverse, 5¢-CGGATCCTTAGGACATGGCAGAG-3¢;DcR2forward,5¢-CGGAATTCCGCGGAAGAAATTCATTTCT-3¢;DcR2 reverse, 5¢-CGGGATCCTCACAGGCAGGACGTAGCAG-3¢; Bax forward, ... activate the caspase cascade. Caspase-8 is known as the initiator caspase in the death receptor signal pathway,while caspase-9 is associated more with the mitochondrialpathway, which is activated ... micrograms of total RNA was reverse transcribed using the TaKaRa OneStep RNA PCR kit (TaKaRa Bio Inc., Shiga, Japan)according to the manufacturer’s instructions. In both RTand PCR steps, the reaction...