... important portion of the fusion protein wasdegraded by the host proteases. On the other hand,attempts to produce a His-tagged version of the Rv1771 protein by using the pRSETc or the GatewaypDEST17 ... followed by concen-tration, the 70 kDa band disappeared, and two newbands of 45 and 26 kDa were seen on SDS ⁄ PAGEthat corresponded to the mycobacterial Rv1771 pro-tein and the freed GST tag, respectively ... 200 mL of culture volume, and then IPTG wasadded to a final concentration of 0.1 mm for induction, and the fusion protein was produced for 1 h or 3 h, as indica-ted. The cells were washed, resuspended...