Báo cáo khoa học: "Optimal management of the high risk surgical patient: beta stimulation or beta blockade" pdf

... included the vast majority of the patients (80% of the cohort). Second, RCRI assessspecifically the cardiac risk of the patient, not the mortalityrelated to the surgical procedure. Accordingly, mortality ... was6% to 7% in the patients with RCRI scores of 3 and more. A high- risk surgical procedure was performed in a minority of the patients, and the effects of beta- blockade were neutral inthese patients.How ... contra-indication for beta- blockade, and the outcome of thesepatients receiving beta- blockade was compared to the otherpatients, using a propensity score to match patients forconfounding factors. In...

Báo cáo khoa học: "Ventilatory management of ARDS: high frequency oscillation and lung recruitment" ppt

... maintain open ≥ 95% of the lung. This required the use of high peak airway pressure (40 to 60 cmH2O) with high PEEPlevels (25 to 45 cmH2O) and the careful selection of the optimal PEEP level ... It is not the mode of ventilation that isimportant, it is the approach used to apply the mode that iscritical! The second issue raised by the Davis and colleagues study[3] is should the lung ... as soon as the patient is hemodynamicallystabilized during the initial application of mechanicalventilation regardless of the mode used. The recruited lungrequires less fraction of inspired...

Báo cáo khoa học: "Highs and lows in high-risk surgery: the controversy of goal-directed haemodynamic management" pps

... only one risk factor hada mortality of 4%, whereas those with two or more risk factorshad a mortality of 20%. The single-centre trials utilize their investigators’ intimateknowledge of the strengths ... have to beinterpreted in the context of the specific institutions wherethey are performed. The risks associated with surgery aremultifactorial, and the same high- risk criteria applied indifferent ... transport-driven guide-lines for peri- and postoperative haemodynamic management versus conventional management. What can be the reasons for these major differences? The major limitations of the Sandham trial...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... lowerthan the metal-containing enzyme.Metal cofactors were incorporated into the enzyme;when the growth medium contained MnSO4 or FeSO4, the activity of the obtained enzymes was 20-fold or six-fold ... plot.Incorporation of metalsMetal cofactors were incorporated into the enzyme by one of two procedures. To incorporate metals during growth of bacterial host cells, 1 mm MnSO4(for Mn-bound ... counted the interactions, we excluded all residuesinvolved in the binding of the metal cofactor.ApeSODs in different forms were superimposed with the least square ﬁt of Ca atoms of the residues...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... RNR and that of other class 1RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E.We consider the ﬁrst option unlikely. Under these cir-cumstances, ... may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is ... amounts of R2F were synthesizedfrom the inserted promotorless nrdF-gene under tightcontrol of the IPTG-inducible tac promotor. This ﬁnd-ing corroborates another study [34] reporting that the hybrid...

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... in the absence of Thr [20]. Therefore, a sim-ilar conformational change is expected for these twoenzymes, depending on the presence or absence of Thr.In CgAK, mutations of the residues in the ... interior of protein, and the richness of Pro residues mainly contribute to the stabilization of TtAK b. This information might be use-ful for the generation of more thermostable CgAKvariants for ... near the Thr-binding site are observed. The mechanism of regulation by Thr is discussedon the basis of the crystal structures. TtAKb has higher thermostabilitythan the regulatory subunit of Corynebacterium...

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... 1. The pCX4bsr expression vectors encodingNS3-4A, NS3, or NS4A from the 1B-1 strain and NS3-4A from the HCV-O strain were used for the transfection. The pCX4bsr expression vec-tor encoding the ... (internal control reporter) and cultured for 42 h, and thenpoly(I-C) (1 lg) was transfected into the cells for 6 h before the reporter assay as described in the Experimental procedures. The relativeluciferase ... cases, 20 ng of pCX4pur ⁄ myc-Cardif or pCX4pur ⁄ myc-TRIF were addedas the effector plasmid. The cells were cultured for 48 h,and then a dual luciferase assay was performed accordingto the manufacturer’s...

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... binds to the ribozyme at a rate expected for simple helix formation. In addition, the rate constant for the ﬁrst step of the TES reaction is more than one order of magnitude lower than the analogous ... that there is room for improve-ment in terms of the rate of reaction. This would bebeneﬁcial not so much in terms of the rate of the over-all reaction, as the cleavage reaction is not the limitingstep ... binds to the ribozymeduring tlis converted to product. Therefore, the amount of product formed during the chase period isrepresentative of the amount of ribozyme–substratecomplex formed during...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... revealsthat the most similar regions of these proteinases medi-ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the ... pocket of DESC1: Asp189–Gln192 (the basement of the pocket), Ser214–Gly219 (the entrance frame), Lys224–Tyr228 (the back of the pocket) and the disulﬁde bridgeCys191–Cys220 (the front of the pocket) ... identicalresidues. The topological equivalence of the fourTTSPs forms the basis for the sequence alignmentshown in Fig. 3. The numbering in the alignmentrefers to the chymotrypsin numbering. Despite the high...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... established by the incorporation of the b8-strand of one monomer into a b-sheet of the other monomer. The position of the zinc ion is indicated by acircle. (B) Schematic diagram of the dimer. Each ... determines the shape of the active site entry. (e) The structure of the active site isessentially identical with the active sites of the MshBand LpxC proteins. The conservation of catalyticallyimportant ... responsiblefor the content or functionality of any supplementarymaterials supplied by the authors. Any queries (otherthan missing material) should be directed to the corres-ponding author for the...

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