... utilize it in theCCh-induced increases in calcium and Ins(1,4,5)P3.Toinvestigate the cell-type dependence of Ins(1,4,5)P3production, we assayed receptor- and calcium-inducedIns(1,4,5)P3increases ... fluorescence inten-sity. Increases in cellular calcium and Ins(1,4,5)P3wereinduced using carbachol (CCh; 100 lm), which specifi-cally activates mAChR and induces Ins(1,4,5)P3pro-duction in PC12 ... protein; Ins(1,4,5)P3, inositol 1,4,5-trisphosphate; mAChR, muscarinic acetylcholine receptor;MDCK cells, Madin–Darby canine kidney cells; PHD, pleckstrin homology domain; PtdIns, phosphatidylinositol;...