báo cáo khoa học: " Spatial distribution of transcript changes in the maize primary root elongation zone at low water potential" docx
báo cáo khoa học: " Spatial distribution of transcript changes in the maize primary root elongation zone at low water potential" docx
... 119:1349-1360.28. Saab IN, Sharp RE, Pritchard J: Effect of inhibition of ABA accu-mulation on the spatial distribution of elongation in the pri-mary root and mesocotyl of maize at low water potentials.Plant ... that distinguished well-watered and water- stressed roots in different regions of the root tip in the hopes of delineating the genetic mechanisms respon-sible for the physiological changes that ... primary root toidentify causes for the changes observed in the spatial pat-tern of root elongation at low Ψw. The two regions of the root studied showed distinctly different transcript profilesunderscoring...
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Báo cáo khoa học: " Spatial variability of humus forms in some coastal forest ecosystems of British Columbia" pdf
... continuity of the data, ie the rela-tionship between the value for a property in one location and the values for the sameproperty at another’location. The spatial continuity ... denotes a set of spatial coordinates in 2dimensions. The measurements give a set of val-ues z(xi), and the semivariance that summarizes the spatial variation for all ... movingaverage trend surfaces for ore evaluation. In: ProcSymp on Mathematics, Statistics, and ComputerApplications in Ore Evaluation. South African Institute of Mining...
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Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt
... obese mice In order to gain insights into the potential biologicallyrelevant role of miR-27 in the regulation of adipose tis-sue functions in vivo, we examined the expression of miR-27 in the genetically ... have investigated the role of miRNAs in the regulation of adipogenic differentiation. In this article, wedemonstrate that the miR-27 gene family is downregulated during adipogenicdifferentiation. ... 3T3-L1 cells were maintained in a hypoxia chamber (Invivo 400; Ruskinn Inc., Cincinnati,OH, USA) constantly maintained at 1% O2. Culturemedium was replaced every other day inside the chamber.For...
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Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf
... showing increased kcatvalues forCDNB, suggesting that Lys117 might positively in uence the formation of the transition state during catalysis. No changes in the Kmvaluestowards glutathione ... the nature of the leaving group, as the sub-stitution of the chlorine atom with the more electro-negative fluorine in the CDNB molecule did not affect the kcatvalues. Altogether, these results ... quantitation of microgram quantities of proteinutilizing the principle of protein-dye binding. AnalBiochem 72, 248–254.40 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of...
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Báo cáo khoa học: Identification of functional domains in the formyl peptide receptor-like 1 for agonist-induced cell chemotaxis doc
... FEBS the involvement of multiple domains of FPR in recogni-tion of the agonist fMLF, including the first, second,and third extracellular loops. The TMs in FPR are alsoimplicated in contributing ... only at concentrations > 100 nm. Theseresults, in addition to the binding data obtained with125I-labeled W peptide, indicate that the chimeric recep-tors are indeed expressed on the surface ... with following corresponding parts in FPRL1: the N-terminus and N-terminal half of the first TM (A: chimera C), the C-terminal half of the first TM through the second TM (B: chi-mera D), the first...
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Báo cáo khoa học: Essential roles of lipoyl domains in the activated function and control of pyruvate dehydrogenase kinases and phosphatase isoform 1 pot
... domains interacting near the base of that side of the wedge outside; the N-terminal domains form the outside of the wedges. The wedge pockets have an extended seamthat extends the length of the interface ... domainand then rebinding to a second mobile lipoyl domain fasterthan releasing from the singly held state [21]. Each of the lipoyl domains of E2 is concentrated within the exterior of the complex ... models of the human L2 domain. The position of Lys173(lipoylated lysine) is at the top of the structures; unstructured regionsthat are not part of the connecting linker regions are shown at the N-...
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Báo cáo khoa học: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt
... activation (in yellow), CoA activation (underlined) andmalate inhibition (in green) of the forwardreaction are highlighted. In addition, the regions involved in succinate activation of the reverse ... concentration in the latter case(Fig. 4).Kinetic characterization of chimeric NADP-MEs in the oxidative decarboxylation direction at pH 7.0 The effect of the substrate l-malate on the forwardreaction ... highlyactivated by aspartate, fumarate and succinate,NADP-ME3 is inhibited by fumarate with no modifi-cation of the enzymatic activity in the presence of aspartate and succinate [5]. Furthermore,...
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Báo cáo khoa học: Kinetic analysis of zymogen autoactivation in the presence of a reversible inhibitor pptx
... straight line, indicating that p-amindinobenzamidineis a c ompetitive inhibitor for the t rypsinogen autoactivationreaction. From the slope and intercept of the straight line, the kinetic parameters ... characterize the effect of inhibitor on the trypsin-catalyzed zymogen activation, the kinetic parameters of trypsin-catalyzed zymogen hydrolysis in the absence of inhibitor were determined first. The ... the slope of the straight line. Therefore, from the change in the s econd-order rate constant of autoactivation in the presence of inhib itor, the KIcan be calculated.However, many of the zymogen-activating...
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Báo cáo khoa học: Identification of a domain in the a-subunit of the oxaloacetate decarboxylase Na+ pump that accomplishes complex formation with the c-subunit pot
... of point mutations in the binding domain of a-2-C on the binding to c¢-2To elucidate whether single amino acids in the bindingdomain of a-2-C were particularly important for the formation of ... of the protein is upstream of the putative linker segmentconnecting the C-terminal biotin-binding domain with the residual parts of a-2 (Fig. 5).For further analyses of the domain of a-2 which ... and the biotin domain. The dynamics of conformational motions within the catalytic cycle of the enzyme probably also includes the motion of the entire soluble part of the enzyme against the mem-brane...
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Báo cáo khoa học: Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases ppt
... subdomains of the A domain. Thesefindings were later explained with the structural modelthat the smaller subdomain AC of the A domainrotates upon substrate binding and changes from anopen conformation ... synthetaseI (TycA) in its apo-form. They found that the addition of the substrates slowed down the proteolysis of the protein, mostly by decreasing the rate of cleavagebetween the two subdomains ... data imply that the 4¢-phosphopantetheine moiety of the peptidyl carrier protein changes its position as a result of a conformational change in the A domain, whichis induced by the binding of...
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