báo cáo khoa học: " New insight into the structures and formation of anthocyanic vacuolar inclusions in flower petals" potx

... dierential scanning cal-orimetry, of bovine b-andj-casein, recombinant human a- ,b-andc-synuclein, together with the A3 0P and A5 3T mu-tants of a- synuclein associated with familial cases of Par-kinson's ... CONCLUSIONSThis study has shown that the casein milk proteins, the brain proteins synuclein and tau, as w ell as mutants of a- synuclein and tau associated with inherited forms of neurodegener-ative disease, ... by a comparative ROA study, supple-mented with DSC, of caseins, synucleins and tau, togetherwith several mutants of a- synuclein and tau that causeneurodegenerative diseases. The ROA spectra...

... thecorresponding OfODC structure [4]. However, theelectron density of the S-acetyl-pantetheine moiety of acetyl CoA was very poor in the correspondingThDP–acetyl CoA EcODC complex.Studies on oxalyl CoA decarboxylase ... catalytic activity of EcODC was in the broadABFig. 6. Spectral changes during decarboxylation of oxalyl CoA. (A) UV ⁄ Vis spectra of oxalyl CoA (solid black line) and formyl CoA (solid dark grey ... catalytic decarboxylation of oxalyl CoA (1, 0 lM;2,10 lM; 3, 16.0 lM;4,35lM;5,50lM)byEcODC (0.26 lgặmL)1)at30 C.T. Werther et al. Studies on oxalyl CoA decarboxylase of E. coli FEBS Journal 277...

... in venoms of many Viperinae [1] and some Elapidae [2,3]. The factor X activator from the venom of Russell’s viper (Daboia russelli and Daboia siamensis) (RVV -X) is a potent procoagulating and ... FEBS 3947 New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom Hong-Sen Chen1, Jin-Mei Chen2, Chia-Wei Lin1, Kay-Hooi Khoo1,2 and Inn-Ho ... 2008)doi:10.1111/j.1742-4658.2008.06540 .x The coagulation factor X activator from Russell’s viper venom (RVV -X) isa heterotrimeric glycoprotein. In this study, its three subunits were cloned and sequenced from the venom gland...

... the measurement of the rate of passive diffusion of the dye through the plasma membrane. This cannot be done by the simplemeasurement of the increase of the fluorescence signal(Fcyto) of the ... proportional to the amount of rhodamine free in the cytosol. This observation allows the further determination of the concentration of drug free in the cytosol of resistantcells. The determination of karequires ... concentration, the rate of their passive diffusionthrough the plasma membrane and then the kineticparameters characteristic of their P-gp-mediated efflux.Our data show that the efficiencies of the P-gp-mediatedefflux...

... for the optimal activity of BsArb43B.Furthermore, to determine whether the role of the calcium is structural, thermal shift assays were per-formed to determine the Tm of the protein in the ... distribution of BsArb43B in the proximity of the active site. Arabinotriose in the binding cleft is representedusing sticks. (B) Detailed view of the binding cleft with arabinotri-ose. (C) Details of the ... 45624574 ê 2010 The Authors Journal compilation ª 2010 FEBS 4563 New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases Daniele de Sanctis1,2,*, Jose´M. Ina´cio1,*,,...

... lacto- peroxidase and horseradish peroxidase mediated nitration of the representative tyrosine derivatives 1–4 by nitrite/ hydrogen peroxide and, in particular, we address the problem of the possible ... °C. The inset shows an expansion of the plot in the low nitrite concentration range.Fig. 2. Inhibition of the LPO-mediated phenol nitration by nitrite. Plot of the rate of LPO -catalyzed nitration ... function of nitrate concentration for the enzymatic nitration of tyrosine derivatives 1–4. Steady-state kinetic parametersdetermined for the LPO and HRP mediated nitration of 1–4 by nitrite and hydrogen...

... bothGCPIII and GCPII, including the ‘glutarate sensor’ and the Pro-rich region [27]. Both GCPII and GCPIIIdisplay an induced-fit movement of the ‘glutaratesensor’ upon binding of a glutamate (or glutamate- like) ... functional and pharmacological over-lap between GCPIII and GCPII, studies addressing the structural and physiological properties of GCPIII are crucial for obtaining a deeperunderstanding of the GCPII ... therapeutics and ligands, as well as in the investigation of the diverse biological functions of GCPIII and GCPII.Experimental proceduresrhGCPIII and rhGCPII expression and purification The extracellular...

... FEBS Sulfated polysaccharides inhibit the catabolism and loss of both large and small proteoglycans in explant cultures of tendon Tom Samiric, Mirna Z. Ilic and Christopher J. HandleySchool of ... to determine the amounts of glycosaminoglycans remaining in the matrix.Measurement of turnover of 35S-labeled proteoglycans in tendon maintained in culture in medium containing varying concentrations ... other cultures. In addition, these data indicate that heparinwas more potent than CaPPS in inhibiting the loss of 35S-labeled proteoglycans from the matrix of tendon explants. The rate of incorporation of...

... betweenZn2+ and Ca2+binding to S100A2 and to addresshow metallation affects the protein conformation and stability, we have investigated the effects of metal ions on the wild-type protein and on ... to the effect of each metal on the stability of each protein mutant (Table 3).Table 2. Thermodynamic stability parameters for the S100A2 variants in the apo, Zn2+(1 : 1) and Ca2+(10 : 1) metallated ... compilation ê 2009 FEBS 1777 Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 Hugo M. Botelho1, Michael Koch2,Guănter Fritz2 and Claudio M. Gomes11...

... associationof FMRP in RNP particles.Thus, despite the fact that the I304N mutation cau-ses Fragile X syndrome, and that investigations of the I304N mutation play a key role in Fragile X research,there ... corresponds to the full-lengthproteins. Genotype vs. pheonotype in Fragile X syndrome I. Pozdnyakova and L. Regan874 FEBS Journal 272 (2005) 872878 ê 2005 FEBS New insights into Fragile X syndrome Relating ... [2,3]. The syndrome takes its name from the physical‘fragility’ of the chromosome. Chromosome breakageoccurs at the tip of the X chromosome at the FRAXAlocus, Xq27.3. The fragility is associated...

... identify silica deposition in horsetail and to provide new insight into silicification in this plant. It was observed that silica deposition in horsetail exactly mirrored the known deposition ... H ART I C L E Open Access New insight into silica deposition in horsetail (Equisetum arvense )Chinnoi Law and Christopher Exley*AbstractBackground: The horsetails (Equisetum sp) are known ... callosewas i nvolved in templating the deposition of silica else-where in horsetail was observed in silica skeletons ofcells undergoing cytokinesis (Figure 5). Again silica deposition at phragmoplasts...

... through promoting the fusion of anthocyanin-containing vesicles into AVI-like structures that contained the spread of anthocyanins from the inclusions into the vacuolar sap. However, light incidence ... participated in the investigation on AVI morphology. K.D. contributed to the initiation of the project and was involved in design and supervision of the research and the writing of the manu-script.AcknowledgementsWe ... CentralPage 1 of 14(page number not for citation purposes)BMC Plant BiologyOpen AccessResearch article New insight into the structures and formation of anthocyanic vacuolar inclusions in flower...

... paucity of evidence tosupport prophylactic chest physiotherapy after abdominalsurgery [15] and other previous negative reviews [16,17].Tsuruta and colleagues report the safety and efficacy of superimposing ... clinicalconsequences of altering the pro-inflammatory cytokineprofile, especially on the incidence of extra-pulmonary organdysfunction, nor indeed am I aware of any evidence tosupport the hypothesis that ... ARDS.Cue the rhythm section?December saw the publication of three papers investigating the therapeutic potential of intermittent intrapulmonarypercussive ventilation (IPV). This technique, and the...