Báo cáo khoa học: "The complete genome of klassevirus – a novel picornavirus in pediatric stool" doc

... The 5' UTR ends with two in- frame AUG A. Genome organization of human klassevirus 1Figure 1 A. Genome organization of human klassevirus 1. Conserved picornaviral domains present in klassevirus ... kv761R 5'-CAG CCA ACG AAC TCG AAAAT-3'. The 761 bp amplicon was gel purified and clonedusing TOPO TA cloning kit (Invitrogen). TOPO TA plas-mid containing the 761 bp insert was sequenced ... 154(1):101-8.5. Yamashita T, Sakae K, Ishihara Y, Isomura S, Utagawa E: Prevalence of newly isolated, cytopathic small round virus (Aichi strain) in Japan. J Clin Microbiol. 1993, 31(11):2938-2943.6. Ambert-Balay...

Báo cáo y học: "The complete genome of klassevirus – a novel picornavirus in pediatric stool" pdf

... of California, San Francisco, California 94143, USA, 2Departments of Laboratory Medicine and Medicine, Division of Infectious Diseases, University of California, San Francisco, California 94143, ... the same adapter-linked oligo-dT primer.454 Pyrosequencing A total of 141 amplified cDNA libraries that were negativeby array and PCR were cleaned via Ampure beads (Agen-court) and quantitated ... 5'-CCC TTT CGA CCGCCT TAT-3' and kv761R 5'-CAG CCA ACG AAC TCG AAAAT-3'. The 761 bp amplicon was gel purified and clonedusing TOPO TA cloning kit (Invitrogen). TOPO TA...

Báo cáo khoa học: "The clinical value of daily routine chest radiographs in a mixed medical–surgical intensive care unit is low"

... insertion of endotra-cheal tubes, intravenous lines and chest drains, but not afterinsertion of nasogastric tubes. In addition, CXRs wereobtained in the case of worsening of oxygenation. As ... clinically relevant and irrelevant findings, and simplyreported on all abnormalities [12]. At present, in many ICUsCXRs are still routinely obtained on a daily basis, at least in TheNetherlands ... design and statistical analysis of thestudy. MS conceived and coordinated the study and wasinvolved in the interpretation of the data and manuscript revi-sion. All authors read and approved...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... other activating factors. The results of employing plasmid-based ChIP assays on the Cdc2promoter indicate that E2F3 binding to the distal acti-vating E2F site may require an intact CCAAT-boxoccupied ... 1). Cyclins B1 and B2 appear in S phaseand accumulate in G2and mitosis before disappearingat the transition from metaphase to anaphase. Synthe-sis is controlled at the level of gene transcription ... contains mammalian homologs of Caenor-habditis elegans synMuvB proteins and is composed of p130, E2F4 ⁄ 5 and DP1 ⁄ 2 and a module containing theMuvB proteins Lin-37, Lin-52, Lin-54 and chromatin-associated...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... life-threatening complications of cerebral malaria, and as an important tool in validat-ing our proposal of hACMSD as a novel drug targetfor the treatment of diabetes and to investigate itsproposed ... Regulation of mouse hepatic a- amino-b-carbo-xymuconate-e-semialdehyde decarboxylase, a keyenzyme in the tryptophan–nicotinamide adenine dinu-cleotide pathway, by hepatocyte nuclear factor 4a andperoxisome ... decarbox-ylase. J Am Chem Soc 129, 927 8–9 279.20 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada H & Shibata K (2002) Identiﬁca-tion and expression of a cDNA encoding...

Báo cáo khoa học: The crystal structure of NlpI A prokaryotic tetratricopeptide repeat protein with a globular fold potx

... 2 0–2 94) were 5¢-aataatccatggggagtaatacttcctggcgtaaaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢.3-TPR NlpI primers (residues 6 2–1 97) were 5¢-aataatccatgggggcacagcttttatatgagcgcggag-3¢ ... structureshave been determined share a common tertiary organ-ization, which is dominated by interactions between A helices and the preceding AB pair (Fig. 1A) . LocalAB, BA¢ and nonlocal AA¢ helix packing ... potential ligand bindingABCDEFFig. 4. Contact map of mature NlpI and packing interactions. (A) Backbone (upper left from diagonal) and side chain (lower right from diago-nal) contacts within...

Báo cáo khoa học: NMR solution structure of Cn12, a novel peptide from the Mexican scorpion Centruroides noxius with a typical b-toxin sequence but with a-like physiological activity doc

... W .A. (1996) Molecular determinants of high a nity binding of alpha-scorpion toxin and sea anemone toxin in the S3–S4 extracellularloop in domain IV of the Na+channel alpha subunit. J. Biol.Chem. ... rectangle the slower the exchange. Arrows indicate b-strands and zig-zaglines indicate the a- helix. (B) Ribbon diagram of Cn12 showing an a- helix at residues 2 4–3 2, three strands of antiparallel ... crayﬁsh, using the same conditions as reported[34].Determination of amino-acid sequence and MS analysisThe full amino-acid sequence of the toxin was obtained bydirect Edman degradation, using...

Báo cáo khoa học: The molecular basis of heme oxygenase deﬁciency in the pcd1 mutant of pea pot

... byphytochrome and a plastid signal during de-etiolation in Arabidopsis thaliana. Plant J 25, 54 9–5 61.54 Sambrook J & Russell DW (2001) Molecular Cloning – a Laboratory Manual. Cold Spring Harbor LaboratoryPress, ... becomes a Val or Leu in mammalian and other animal ⁄ bacterial sequences,respectively, Ile214 is a Leu in cyanobacterial and ani-mal HOs, Tyr231 is a Phe in animal sequences andSer274 is always ... 5¢-GAG GAN ATG AGN TTN GTN GCNATG AGA-3¢ and PS1.REV 5¢-CCA CCA GCA NTATGN GNA AAG TAG AT-3¢. Ampliﬁcation products wereligated into pCR2.1 (TOPO TA cloning kit; Invitrogen Ltd,Paisley, UK) and...

Báo cáo khoa học: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells" doc

... telomerase activity, induction of reactive oxygen species release, and inhibi-tion of survival pathways involving extracellular signal regulated kinase, Akt, calcium signaling and NF-κB activ-ities ... conformational change, Bax acti-vation and up-regulation of total Bax expression involved arsenic trioxide-induced apoptosis rather than affecting total Bcl-2 expression in human gastric cancer ... toxic, it has been shown to have a therapeutic potential. It has for long been used as a drug in traditional Chinese medicine to treat a variety of dis-eases, including malaria, psoriasis, syphilis,...

báo cáo khoa học: "The geographical distribution of P-M hybrid dysgenesis in Drosophila melanogaster" ppt

... d Harwich (P). In North Africa, Europe and Asia the distribution patterns change dramatically,P strains being almost absent and M strains very common. In fact, a marked ... other main area where P strains hasbeen found is Central Africa from Senegal to Kenya. In these regions M strains alsoappear rare and the observation of a relatively ... thusprobably representing the same population. In all other regions from Yugoslavia toJapan, a majority of M strains has been characterised, generally with a high level of GD...

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