Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
... attribute.Isolation and cloning of cotton rat cells expressing human co -receptors for HIV- 1Figure 1Isolation and cloning of cotton rat cells expressing human co -receptors for HIV- 1. (A), VCRT C17; ... assem-bled and released from cotton rat cells. Detection of episomal and integrated HIV- 1 cDNA in infected cotton rat cells In order to detect the presence of HIV- 1 episomal cDNA in infected cotton rat ... to enhance HIV- 1 entry, DNA integration, and production of infec-tious viral particles in cotton rat cells. ResultsIsolation of cotton rat cells expressing human CD4 and HIV- 1 co -receptors To...
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Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx
... attribute.Isolation and cloning of cotton rat cells expressing human co -receptors for HIV- 1Figure 1Isolation and cloning of cotton rat cells expressing human co -receptors for HIV- 1. (A), VCRT C17; ... assem-bled and released from cotton rat cells. Detection of episomal and integrated HIV- 1 cDNA in infected cotton rat cells In order to detect the presence of HIV- 1 episomal cDNA in infected cotton rat ... to enhance HIV- 1 entry, DNA integration, and production of infec-tious viral particles in cotton rat cells. ResultsIsolation of cotton rat cells expressing human CD4 and HIV- 1 co -receptors To...
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báo cáo khoa học: " Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells" potx
... specific for cytotoxic cells. It would be interesting to determine ifthis behavior is a more general property of MICA- and MICB-producing cells by evaluating whether virus-infected and tumor cells ... (Figure 6).CALO and INBL proliferate in response to MICA and MICBAfter we detected the expression of MICA, MICB, and NKG2D in CALO and INBL cells, we proceeded to eval-uate if MICA and MICB could ... nm.Immunohistochemical analysis of NKG2DImmunohistochemical staining for the expression of NKG2D was co mpleted by standard procedures. In brief, CALO and INBL cel l lines were seeded onto poly-L-lysine-coated...
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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
... determination.RESULTSTransformation and screening In a cotransformation experiment, A. niger D1 5#26 wastransformedwithamixtureofplasmidpAB4-1andeachofthe t wo expression vectors c ontaining ... ABTS and syringaldazine.Effect of temperature and pH on the laccase activity.Purified laccase (100% refers to 0.5 and 0.8 U ÆmL)1,respectively, using ABTS and syringaldazine as substrate)was ... mgÆL)1 of wild-type laccase from thedikaryotic strain I-937 of P. cinnabarinus and 145 mgÆL)1from the derived monokaryotic strain ss3 of P. cinnabarinus. In order to improve the secretion of the...
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báo cáo khoa học: "Dysregulation of miR-15a and miR-214 in human pancreatic cancer" potx
... cultures and clinical samplesBxPC-3 human pancrea tic cancer cells were maintained in RPMI 1640 medium containing 10% fetal bovineserum (FBS; Gibco BRL). 293T cells were maintained in DMEM containing ... advancedpancreatic cancer, and i t acts by inhibiting tumor cellproliferation and inducing a poptosis [22-25]. Prior todetermining the effects of GEM on miR-214, weTable 1 Expression of miRNAs in pancreatic ... equally1Key Laboratory of Gene Engineering of the Ministry of Education, State KeyLaboratory for Biocontrol, Sun Yat-sen University, Guangzhou 510275, PRChinaFull list of author information is...
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Tài liệu Báo cáo khoa học: "Applications of GPC Rules and Character Structures in Games for Learning Chinese Characters" doc
... in naming Chinese characters, Language and Linguistics, 6(1), 75–107. C H. Lin and P. Collins. 2012. The effects of L1 and ortho-graphic regularity and consistency in naming Chinese char-acters. ... formation of Chinese characters, and radicals carry partial semantic information about Chinese charac-ters. Hence, one may use radicals as hints to link the meanings and writings of Chinese characters. ... approaches and designs mainly employ visual stimuli in activities. We re-port exploration of using the combination of audio and visual stimuli. In addition to pictograms, more than 80% of Chinese...
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Báo cáo khoa học: Roles of the SH2 and SH3 domains in the regulation of neuronal Src kinase functions pptx
... results in blocking of the binding of the SH2 domain to thesubstrate and thereby preventing interaction of the substrate with the kinase domain. For active n-Src in which the C-tail tyrosine was ... demonstrated that intra-domain interactions betweenSrc family kinases (SFKs), stabilized by binding of the phosphorylatedC-terminus to the SH2 domain and ⁄ or binding of the SH2 kinase linker ... 400[nM]Fig. 5. Binding of n-Src and NR2A C-tail proteins. (A–F) Surface plasmon resonance showing binding of wild-type and mutant n-Src proteinsat concentrations of 0–400 nM to NR2A C-tail protein immobilized...
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Báo cáo khoa học: Role of glutaredoxin 2 and cytosolic thioredoxins in cysteinyl-based redox modification of the 20S proteasome docx
... fragmentation using massspectrometry, by incubating Grx2 in standard buffer for 30 min or 2 h in the presence of n-PT. After incu-bation, standard Grx2 and fragments recovered by fil-tering the incubation ... of proteasomepreparations with cardiolipin and Grx2. The resultsobtained showed 25% and 65% increased chymotryp-sin-like activity and 61% and 100% increased post-acidic cleavage of n-PT and ... preparations were used for further incubation, immunoblot analyses, SDS–PAGE and hydrolytic assays.Cloning and expression of yeast GRX2Cloning of the yeast GRX2, its expression in E. coli, and Grx2...
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Báo cáo khoa học: Function of microRNA-375 and microRNA-124a in pancreas and brain doc
... improvecytoskeleton organization and regulate glucose-inducedinsulin secretion [65,66]. These findings representanother interesting aspect of the action of myotrophin in cells and may explain the mechanism ... RNaseIII-type protein, Dicer,which acts in concert with another double-strandedRNA-binding protein (the HIV transactivatingresponse RNA-binding protein) and Argonaute pro-teins to liberate the mature ... in the differentiation of progenitor cells to mature neurons by directlyregulating polypyrimidine tract-binding protein 1, whichis involved in alternative pre-mRNA splicing in non-neural cells...
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Báo cáo khoa học: "Overexpression of cyclin D1 and cyclin E in 1,2-dimethylhydrazine dihydrochloride-induced rat colon carcinogenesis" potx
... PCNA. Cyclin D1 expression was detected in 56.3% of the adenomas and in 61.5% of the adenocarcinomas examined, whereas cyclin E expression was detected in 87.5% of the adenomas and in 92.3% of the ... wasalso increased 1.9-fold in adenomas and 1.8-fold in adenocarcinomas (p<0.05). Immunohistochemical stainingrevealed exclusive nuclear staining of the neoplastic cells for cyclin D1, cyclin E and ... daeyong@plaza.snu.ac.krCyclin D1 and E expression in rat colon tumor 125significant difference in either the mRNA levels or proteinexpressions of cyclin D1 and cyclin E in adenomas and adenocarcinomas. These...
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