Báo cáo khoa học: " Characterization of a VHS virus genotype III isolated from rainbow trout (Oncorhychus mykiss) at a marine site on the west coast of Norway" doc
Báo cáo khoa học: " Characterization of a VHS virus genotype III isolated from rainbow trout (Oncorhychus mykiss) at a marine site on the west coast of Norway" doc
... Access Characterization of a VHS virus genotype III isolated from rainbow trout (Oncorhychus mykiss) at a marine site on the west coast of NorwayHenrik Duesund, Stian Nylund, Kuninori Watanabe, ... CCC ATG AGC CGC TCT CTC TElongation factor EL 1A- elaf CCC CTC CAG GAC GTT TAC AAA1 alpha EL 1A- elam1 ATC GGT GGT ATT GGA AC 57 nt [45]S. salar EL 1A- elar CAC ACG GCC CAC AGG TAC A Duesund et al. ... trout (Oncorhychus mykiss) using a Viral haemorrhagic septicaemia virus (VHSV) isolate, genotype III, collected from farmed rainbow trout at a marine site on the west coast of Norway. Master thesis...
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Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx
... concentration was kept at 850 lMandtheNAD(P)Hconcentrationvariedfrom0to300lM(Fig. 3A, B). The data were fitted to a hyperbola (Eqn 3) to estimate the catalytic constant (kcat)and Km.m½E0¼ ... 14 h at 4 °C, kinetic experiments showed that the kcat of the reconstituted complex was still equal to the kcat of native GAPDH and the K0.5for BPGA also became equalto that of native GAPDH. ... Mean rate constants and standard deviationsare reported as a function of NADPH concentration in the assaycuvette. The points were fitted to a hyperbola (Eqn 3 in the main text).(B) The NADH-dependent...
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Báo cáo khoa học: Characterization of L-aspartate oxidase and quinolinate synthase from Bacillus subtilis potx
... the oxidation of l-aspartateto iminoaspartate; the second enzyme, quinolinate syn-thase (NadA), is encoded by the gene nadA and cata-lyzes the condensation between iminoaspartate andDHAP, resulting ... of iminoaspartate. The iminoaspartate itself was stabilizedupon binding to NadB, as the observed rate constant of hydrolysis of free iminoaspartate to oxaloacetate andammonia under these conditions ... Iminoaspar-tate was produced by an enzymatic system consisting of D-aspar-tate ⁄D-aspartate oxidase to produce iminoaspartate in situ free of excess reagents, using a concentration of D-aspartate...
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... from clones isolated previously[22] using the following primers: gatggatcccatATGGGTGTTGAAGTTGTA annealing around the start codon of the Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGCannealing just ... dependence of PPI1-induced activation of the H+-ATPase showed thatstimulation decreases dramatically with the increase of pH above pH 6.8; PPI1-induced activation of the H+-ATPase becomes ... that stimulation of the H+-ATPase activity in PM isolated from controlor FC-treated cells similarly increased with the increase of PPI1588His6concentration; the k0.5values evaluatedfrom...
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Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt
... 5¢-GAAAAACCATGGTGAATGAATACCAAGCGACT-3¢ ) anddownstream primer (NP7: 5¢-CAATCTCCATGGCTAGTAGCTTGCACTCAG-3¢) containing a NcoI restriction site and 1 U of Pfu-polymerase. PCR amplification was carriedout at ... the enzymatic activitiesoriginating from these strains (unpublished data).One of the marine bacteria showing peptidase activ-ity was closely related to Serratia proteamaculans of the Serratia ... 5¢-GATGAAAATCCTAACCTCTCCCCCGCACAG-3¢; OP7, 5¢-ACTGCACCTACGGCGGGTCGTTGGTACGTG-3¢; NP4, 5¢-GACACCGTAGGTTGAGCCGCCAATCGTCCC-3¢; NP5, 5¢-CTTTAACTTGTTGGGCACTGGCATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCCCA-3¢...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... sequence aaATGCa to aaTTCCa (core bases shown in uppercase letters)centered at )123 within Prm3 was performed using the mutator primers Kin175 (5¢-CACCAGAGCTACTTACACTGAATTCCAGAATAATCACAAGCAAATC-3¢; ... 5¢-dGAGAGGTACCGAATTAATCACAAGCAAATCTTCTC-3¢, corresponding to NTs )119 to )94).(7) Prm3aab; pGL3b:Prm3aab & pGL3e:Prm3aab. (PrimerKin160; 5¢-dGAGAGGTACCGCAAATCTTCTCTCGCCTCC-3¢, corresponding ... alpha and beta isoforms. Bio-chim Biophys Acta 1425, 543–559.25 Coyle AT, Miggin SM & Kinsella BT (2002) Characteri-zation of the 5¢ untranslated region of alpha and betaisoforms of the...
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Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf
... M. brassicae CSPMbraA6[32]. We observed also that BrC15-Ac was able to displaceASA, suggesting that brominated fatty acid and ASA bothassociated with W81 in the same ligand binding site. The ... revealed that ASP3c mainly consists of a- helices, like other insect CSPs. Gel filtration analysisshowed that ASP3c is monomeric at neutral pH. UsingASA, a fluorescent fatty acid anthroyloxy analogue ... maximal percentage of displacement reached at high ligand concentration; IC50, ligand concentration provoking a decay of fluorescence of half-maximal intensity; Kdiss,apparentdis-sociation...
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Báo cáo khoa học: Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae potx
... alternative pathways are avail-able, it is unlikely to play any significant role in the catabolism of the branched-chain amino acids or of methionine.Identification and mutation of residues affectingsubstrate ... the Bradford assay using BSA as standard.Assay The decarboxylation activity of ScPPDC-His on a range of aromatic and aliphatic 2-keto acids was monitored at 30 °Cby a coupled assay described ... E545Lvariant precipitated in this buffer and therefore was assayedunder the standard conditions used for all other substrates,although the reaction was monitored at 366 nm. Characterization of...
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Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt
... a- tocopheroleacetate (1 mgÆmL)1) was added as internal standard to eachassay prior to extraction. The conversion rates weredetermined by calculating the decrease of substrate peakareas measured at their ... anexplanation for the impact of lycopene accumulation on the emission of geranial, as observed in the fruits of several tomato and watermelon varieties [46], as wellas in transgenic tomato fruits, ... bond combination was only detectable inlonger incubations (data not shown). GC-MS analyses of the lycopene incubations demonstrated the forma-tion of pseudoionone and 6-methyl-5-hepten-2-one.However,...
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Báo cáo khoa học: Characterization of inhibitors of phosphodiesterase 1C on a human cellular system potx
... GATCATGCACTGAAATTTA (2), GATGAAACCTCTCAAACTG (3), and CATCATCGCTGGACAATGT (4)], usingT. R. Dunkern and A. Hatzelmann Characterization of inhibitors of PDE1CFEBS Journal 274 (2007) 4812–4824 ª 2007 The ... ionomycin at these higher concentra-tions. The addition of 50 mm EGTA (as a control) at the end of the experiment (indicated by a solid arrowin Fig. 4A) decreased the Ca2+signal. On the basis of these ... lL of a substrate solution (Promega, Madison, WI,USA; CytoTox96 assay), and after 15 min of incubation,an additional 50 lL of a stop solution. Thereafter, extinctionwas measured at a wavelength...
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Từ khóa: Nghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015QUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ