Báo cáo khoa học: "Molecular characterization of the virulent infectious hematopoietic necrosis virus (IHNV) strain 220-90" doc

... in other IHNV strains. The first 15 of the 16 nucleotides at the 3’- and 5’-termini of the genome are complementary, and the first 4 nucleotides at 3’-ends of the IHNV areidentical to other ... Vakharia1*AbstractBackground: Infectious hematopoietic necrosis virus (IHNV) is the type species of the genus Novirhabdovirus,within the family Rhabdoviridae, infecting several species of wild and hatchery ... 7:10http://www.virologyj.com/content/7/1/10Page 9 of 11RESEARC H Open AccessMolecular characterization of the virulent infectious hematopoietic necrosis virus (IHNV) strain 220-90Arun Ammayappan1,2, Scott...

Báo cáo khoa học: "Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) isolate from USA" pot

... members of the Novirhabdovirus genus. The termini of the viral genome have conserved sequences at the 3'-end (CAUAG/UU) and 5'-end (G/AAUAUG) asother members of the Novirhabdovirus genus. ... Novirhabdovirus genus. The first 4 nt of the leader sequence VHSV are complementary to the last 4 nt sequence of the trailer region (Fig 2B). The length of the 3' leader of MI03GL is 53 nts, ... yellow net virus; SVC, Spring viremia of carp virus; Taro vein chlorosis virus (Taro); VSV, Vesicular stomatitis virus; IHNV, Infectious hematopoietic necrosis virus; HIRRV, Hirame rhabdovirus;...

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... pathways. Neitherendocytic (Fig. 6) nor biosynthetic transport to the vacuole (Fig. 7) is affected in the absence of Osh6p or the presence of a high level of Osh6p. The discrepancymay be due to the ... charac-terization of Osh6p, one of the seven OSBP homo-logs in yeast. We show, for the ﬁrst time, a directbinding of PA by the ORD domain and the cellularlocation of Osh6p. We further demonstrate ... limiting membrane of late endosomesinvaginates and buds into the lumen of the organelle,and is responsible for the biosynthetic delivery of lyso-somal hydrolases and the down-regulation of numerousactivated...

Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx

... amolecular mass of 53 kDa and a pI of 7.5 (Fig. 2A),corresponding to the predicted size of the putative pro-tein calculated from the HlSCP1 amino acid sequence.To detect the localization of endogenous ... occurs in the food vacuole, where plasmepsin,a member of the aspartic protease family, initiallycleaves in the conserved hinge region of the Hb alphachains [49,50]. Falcipain, a member of the cysteineprotease ... part in the host’s blood-digestion process. The protease cascadefor Hb digestion would be crucial for the survival of ticks, for which the blood-meal from the host is the only source of energy....

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identiﬁed in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position in the ... inﬂu-enced either by nucleotides outside of the consensusmotif or by small variations within the consensus. The interaction of APUM-2 with the FAS-2 tran-script was the strongest among the interactions ... in the interactions between APUM-2 and the FAS-2 transcripts with substitutions in the nucleotides down-stream of the APBE. (D) Quantitative analysis of LacZ activation in the interactions of...

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... between the twosteps of the TES reaction The substrate guanosine to be excised (G1) and its2¢-OH group are required for the second step of TES [48], similar to (if not the same as) the role of the ... that there is room for improve-ment in terms of the rate of reaction. This would bebeneﬁcial not so much in terms of the rate of the over-all reaction, as the cleavage reaction is not the limitingstep ... of the xG in the second step of self-splicing [48–56].This suggests that the guanosine to be excised islikely binding to the GBS of the ribozyme for the exon-ligation step of TES. In the substrate-cleavagestep,...

Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx

... ligaseand the role of Pin1. Pin1 is brought in with the cyclin E substrate, through interactionwith the pSer384-Pro motif. When the com-plex contains the CDC4a isoform, the iso-merase activity of ... for destabilizing the double bond character of the pThr-Pro bond [26].Further evidence is available for the catalytic mecha-nism of Pin1 resembling more closely that of the otherprolyl cis ⁄ ... whereas in the study on cyclin E degradation, Pin1 was brought in by the phospho-cyclin E and not by the CDC4a compo-nent of the ﬁnal complex (Fig. 2) [35]. Therefore, the outcome of the molecular...

Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx

... explained by the observation that the amount of H2O2measured, was inﬂuenced by the time period between the NADH conversion and the actual measurement of H2O2. Apparently, the amount of H2O2in ... on the absolute activity,DTT also raised the stability about twofold (half-life83 min) (Fig. 7). The product of the NADH oxidase reactionNADH oxidases can perform the bivalent reduction of oxygen ... electronacceptors (Table 3). However, none of these caused anoxidation of NADH when added to the anoxic reactionmixture.Discussion The noxA-1 and the noxB-1 gene from the hyperthermo-philic archaeon A. fulgidus...

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identiﬁes a calcium sensitive region required for basal activity doc

... 2002 The understanding of the fine tuning of MGP geneexpression requires further investigation and the use of different vertebrate systems may be useful in bringing newinsights into the mat ter of ... identified 5¢ end of the xMGP cDNA [4]. The lower group of bands, identified as site ÔBÕ in Fig. 4,probably corresponds to a premature arrest of the reversetranscriptase due to the presence of an inverted ... copies of the )70/)36regulatory sequence led to a nearly 400-fold induction of reporter gene e xpression, further c onfirming the i mportance of the regulatory element for xMGP gene expression.These...

Báo cáo khoa học: Molecular characterization of a novel nuclear transglutaminase that is expressed during starﬁsh embryogenesis ppt

... (S) or the immunoprecipitates (IP) wasmeasured, and is expressed as the percentage of the total activity in the 10 lL of concentrated Sup4 (Input). The results shown are the aver-ages of three ... localization of the expressed protein was moni-tored. The results, as shown in Fig. 8B, clearly demonstrate the ability of N57 to promote the nuclear accumulation of PK (Figs 8B, d,h). Without N57, the ... t he TG of vertebrates and arthropods[34]. Its molecular mass is within the 75–90-kDa rangeknown for the TG of these organisms [34]. The most uniqueproperty of nTG, not found in other TGs,...

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