Báo cáo khoa học: "Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) isolate from USA" pot

... of the Novirhabdovirus genus. The termini of the viral genome have conserved sequences at the 3'-end (CAUAG/UU) and 5'-end (G/AAUAUG) asother members of the Novirhabdovirus genus. The ... and codingregions of several other strains of VHSV have been deter-mined later [10]. In this study, we characterized the entiregenome of the Great Lakes VHSV isolate MI03GL from muskellunge, ... about the viral hemorrhagic septicemia virus (VHSV) isolates used in this study for comparison and phylogenetic analysis (Continued) the DNA sequenced (Fig. 1). The complete genomesequence of...

Báo cáo khoa học: "Molecular characterization of the virulent infectious hematopoietic necrosis virus (IHNV) strain 220-90" doc

... in other IHNV strains. The first 15 of the 16 nucleotides at the 3’- and 5’-termini of the genome are complementary, and the first 4 nucleotides at 3’-ends of the IHNV areidentical to other ... 134:207-220.17. Ammayappan A, Vakharia VN: Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) strain from USA. Virol J2009, 6:171.18. Nicholas KB, Nicholas ... other rhabdoviruses, the genomic termini of IHNV 3’-terminal nucleotides exhibit complementaritiesto the nucleotides of 5’ -terminu s of the genomic RNA(Fig. 2B). The complementary nature of...

Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx

... of note that the structure of the LPS O-antigen of F. columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21],which is a linear polymer of ... However, the nature of the adhesinsinvolved have not been identified, but possible candidates areLPS, capsule, fimbriae or other appendages of the bacterium,a hypothesis requiring further investigation.It ... chemical shift assignments in the 1H-NMR and13C-NMR spectra and the characterization of the glycosecomponents in the O-PS were determined from the appli-cation of COSY, TOCSY, NOESY and1H,13C-HSQC...

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... pathways. Neitherendocytic (Fig. 6) nor biosynthetic transport to the vacuole (Fig. 7) is affected in the absence of Osh6p or the presence of a high level of Osh6p. The discrepancymay be due to the ... charac-terization of Osh6p, one of the seven OSBP homo-logs in yeast. We show, for the ﬁrst time, a directbinding of PA by the ORD domain and the cellularlocation of Osh6p. We further demonstrate ... limiting membrane of late endosomesinvaginates and buds into the lumen of the organelle,and is responsible for the biosynthetic delivery of lyso-somal hydrolases and the down-regulation of numerousactivated...

Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx

... the blood-meal from the host is the only source of energy. Elucidation of the function of HlSCP1 in the hard tick may contribute a betterunderstanding of the physiology of blood digestionand ... amolecular mass of 53 kDa and a pI of 7.5 (Fig. 2A),corresponding to the predicted size of the putative pro-tein calculated from the HlSCP1 amino acid sequence.To detect the localization of endogenous ... occurs in the food vacuole, where plasmepsin,a member of the aspartic protease family, initiallycleaves in the conserved hinge region of the Hb alphachains [49,50]. Falcipain, a member of the cysteineprotease...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identiﬁed in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position in the ... elegansmRNA database, they found a high number of mRNAs that have the binding motif on their 3¢ UTRsequence. Some of the mRNAs were shown to bepotential targets of the PUFs homologs in a three-hybrid ... inﬂu-enced either by nucleotides outside of the consensusmotif or by small variations within the consensus. The interaction of APUM-2 with the FAS-2 tran-script was the strongest among the interactions...

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... between the twosteps of the TES reaction The substrate guanosine to be excised (G1) and its2¢-OH group are required for the second step of TES [48], similar to (if not the same as) the role of the ... slower than the Tetrahymena ribozyme.Therefore, it appears that there is room for improve-ment in terms of the rate of reaction. This would bebeneﬁcial not so much in terms of the rate of the over-all ... of the xG in the second step of self-splicing [48–56].This suggests that the guanosine to be excised islikely binding to the GBS of the ribozyme for the exon-ligation step of TES. In the substrate-cleavagestep,...

Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx

... ligaseand the role of Pin1. Pin1 is brought in with the cyclin E substrate, through interactionwith the pSer384-Pro motif. When the com-plex contains the CDC4a isoform, the iso-merase activity of ... for destabilizing the double bond character of the pThr-Pro bond [26].Further evidence is available for the catalytic mecha-nism of Pin1 resembling more closely that of the otherprolyl cis ⁄ ... samegroup examined in great detail the structure of bothcis and trans conformers of the same peptide by NMRspectroscopy [31]. The local structure of the cis con-former of this peptide is characterized...

Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx

... potential electronacceptors (Table 3). However, none of these caused anoxidation of NADH when added to the anoxic reactionmixture.Discussion The noxA-1 and the noxB-1 gene from the hyperthermo-philic ... group of small 20-kDa proteins, thatform H2O2instead of H2O. The NADH oxidases from Thermus aquaticus and Thermus thermophilus also belong tothis group [7,8]. The physiological role of these ... hypothesis certainly deserves further attention, butrequires puriﬁcation of the rubredoxin and neelaredoxin. The production of H2O2is in contrast with the data onmesophilic homologues of...

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identiﬁes a calcium sensitive region required for basal activity doc

... analysis of the 5 ¢ end of the xMGP promoter reveale d a minimal activating ele-ment in the s equence from )70 to )36 bp. Synthetic reporterconstructs containing three c opies of the d efined ... to the right of each column. The data show a repre-sentation o f five independent experiments.Fig. 7. Binding of a nuclear factor from A6cells to the )70/)36 region of the xMGPpromoter. The ... 2002 The understanding of the fine tuning of MGP geneexpression requires further investigation and the use of different vertebrate systems may be useful in bringing newinsights into the mat ter of...

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