... by lysing cells in M2buffer [ 20 mmol /L Tri s-HCl (pH 7. 6), 0.5% NP40,250 mmol /L NaCl, 3 mmol /L EDTA, 3 mmol /L EGTA,2 mmol /L DTT, 0.5 mmol /L phenylmethylsulfonyl fluor-ide, 20 mmol /L b-glycerophosphate, ... effect on cisplatin-induced cancer cell cytotoxicity.Methods: Two cervical cancer cell lines, HeLa and Siha, an ovarian cancer cell line, SKOV3, and a non-small celllung cancer cell line, A549, ... described in Fig. 1A.Figure 3 Saikosaponins induce intracellular ROS accumulation in HeLa cells. HeLa cells were treated with cisplatin (8 μM) orsaikosaponin-a (10 μM) or saikosaponin-d (2 μM) individually...