... volume of 100 lL containing 2 mm MgCl2,40mml-glutamate and 50 mm Tris buffer, pH 8.5. The Km and kcatfor Ado-Cbi were determined in the presence of 10, 25, 50, 75 and 120 lm cofactor, and ... AdoCbi and AdoCbi-GDP were alsoinvestigated. Here, we report the results of coenzyme-binding and kinetic studies of AdoCbl analogs with glutamate mutase.ResultsSynthesis of MeCbi, AdoCbi and ... analog bindingcurves with a fixed concentration of glutamate mutase.AdoCbl, MeCbi, AdoCbi and AdoCbi-GDP werebound with apparent Kdvalues of 3.7 ± 0.5,6.0 ± 0.9, 18 ± 3 and 14 ± 3 lm, respectively(Fig....