báo cáo khoa học: " Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine" ppt

... can-didate acyltransferases. However as one of the Arabidopsis enzymes turned out to have strong dual activity with highLPAAT activity, we also decided to express the Arabidopsis acyltransferases ... of microsomal protein added to the assays of the ale1 transformants were: for LPS, 1.88 μg, LPC and LPE, 1.33 μg, and LPA, 1.16 μg, and in the assay of LPA as an acceptor in the slc1 transformants, ... http://www.biomedcentral.com/1471-2229/9/60Page 3 of 8(page number not for citation purposes)Assays with 16:0-LPE and 18:1-LPE with different combi-nations of 16:0-CoA and 18:1-CoA showed that both Ara-bidopsis acyltransferases...

Báo cáo khoa học: Characterization of a high-afﬁnity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt

... 2003fraction of S. oryzae WAA42 was then used for further characterization, with 1–3 lg of protein loaded per assay,in order to work in the linear part of the curve.The proteinaceous origin of the ... °C and used within amonth after labeling. For iodination of the peptide using nonlabeled iodine, theprotocol was as described above, except that 200 lgofthe3741 Da isoform, 0.3 mg of nonlabeled ... aliquots (2 lg of proteins) were incubated with eﬀectors for 10 min at 37 °C before measuring the bindingactivity using 0.4 nM of 125I-labelled PA1b.bIncubation in bindingbuﬀer without antiproteases...

Báo cáo khoa học: Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes pptx

... using 2 mL of 0.1Msodium methoxide for 5 minat 90 °C. Preparation of methyl esters from the seeds wereperformed by treatment of whole seeds with 2 mL of 0.1Msodium methoxide for 55 min ... transformed with the D6acetyl-enase gene.It was of particular interest to investigate the stereochem-istry in the removal of hydrogen atoms in the formation of the two geometrical isomers of ... signiﬁcant increase of 18:2D9Z,12Zin the fad2 line transformed with CREP1 compared with thenontransformed mutant, indicating that the acetylenase alsocould carry out D12(Z)desaturationofoleate.We...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... exon 2 of the DNASE1. Conditions for these ampliﬁ-cations were 95 °C for 9 min, 30 cycles of 94 °C for 1 min,60 °C for 1 min, and 72 °C for 2 min, followed by incuba-tion at 72 °C for 10 min. ... mixture with 6 lL of Lipofectamine Plus reagent at room temperature for 10 min. Four microliters of Lipofectamine Plus reagentwere diluted in 100 lL of Opti-MEM I-reduced serum med-ium. The two ... respectively. Con-ditions for these ampliﬁcations were 95 °C for 9 min, 37cycles of 94 °C for 1 min, 65 °C for 1 min, and 72 °C for 2 min, followed by incubation at 72 °C for 10 min. The PCRproducts...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... interaction partner for the C-termi-nus of Arabidopsis thaliana plasma membrane H+-ATP-ase (AHA1 isoform): site and mechanism of action onH+-ATPase activity differ from those of 14-3-3 pro-teins. ... activation of Fig. 2. Stimulation of A. thaliana PM H+-ATPase activity as a func-tion of the concentration of His6PPI188and PPI1588His6. PM treat-ment with the speciﬁed concentrations of ... truncated of its ter-minal 24 hydrophobic amino acids with a His6-tag at itsC-terminus (PPI1588His6) was transformed into BL21(DE3)Fig. 7. Schematic model of the mechanism of action of PPI1...

Báo cáo khoa học: Characterization of c-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana pptx

... Vmax/Km-quotient was twofold higher for d- than for c-tocopherol.Kinetics of Arabidopsis TMTThe following kinetic analysis was performed for theforward reaction in the presence and absence of inhibitors.Substrate ... herewe performed a detailed biochemical characterization of ac-tocopherol methyltransferase (c-TMT) from Arabidopsis thaliana and of a c-TMT puriﬁed from Capsicum annuumfruits, a tissue with high ... similar values for the pepperenzyme. For Arabidopsis c-TMT a more than twofoldhigher value was deduced for d-tocopherol relative toc-tocopherol indicating a higher catalytic efﬁciency for thissubstrate.Initial...

Báo cáo khoa học: Characterization of scorpion a-like toxin group using two new toxins from the scorpion Leiurus quinquestriatus hebraeus doc

... lM,therelativeamplitude of the maintained current was always higher for hNav1.5 (10–19% of the peak) than for rNav1.4 (2–6% of the peak). The order of potency of toxins was the same for the two muscular ... therelease of histidine upon digestion of the toxin with carboxypeptidase A.Comparison of primary structures of representativea-mammal, a-insect and a-like scorpion toxins with thesequence of the ... Kd of 3.44 ± 0.5 nM(n ¼ 3) andaBmax of 6 ± 3 pmolÆmg)1 of protein. The afﬁnity of Lqh6is in accordance with its Ki(4.35 ± 0.8 nM)andthecapacity of its binding sites is in the range of...

báo cáo khoa học: " Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis thaliana" pdf

... Knowledge of the structure of mutated DNA will contribute to the effective exploitation of heavy-ion beam mutagenesis. Results: Dry Arabidopsis thaliana seeds were irradiated with carbon (C) ions with ... total volume of 10 µl with PCR-grade water. The reaction conditions comprised an activation step at 95°C for 10 min followed by 50 cycles of 95°C for 10 s, a touchdown of 65°C to 55°C for 10 s ... measurements of the higher yield of short DNA fragments after high-LET irradiation in both animals and plants [20,46]. Therefore, it is likely that the quality of DSBs induced by C ions with LET of 30.0...

báo cáo khoa học: " Characterization of WRKY co-regulatory networks in rice and Arabidopsis" ppsx

... 12Co-regulatory networks of Arabidopsis WRKY genes. Co-regulatory networks of Arabidopsis WRKY genes obtained with the PCC threshold value of 0.7 in the untransformed (P-lin) (panel A) and log transformed ... 4Co-regulatory networks of Arabidopsis WRKY genesFigure 4Co-regulatory networks of Arabidopsis WRKY genes. For each pair of WRKY genes, the Pearson Coefficient was calcu-lated on untransformed values ... through two layers of gauze.Inoculations with Magnaporthe strains were performed with rice plantlets 2 weeks after sowing by spraying with conidial suspensions. Thirty mL of either a 100,000 (for FR13)...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... deconvolution of spectra of the typeshown in Fig. 1A¢, with ML signals of a very lowintensity, was performed as a ﬁrst step. Deconvolution of spectra of the type shown in Fig. 1A, with intenseML ... apoptosis of tumour cells with the level of reduced glutathione, as detected by1H NMR [14].These cells were therefore considered for use in mag-netic resonance spectroscopy analysis for the ... otherstudies have not [8,9].A number of studies have been performed in view of the possible use of MLs as spectroscopic markers of cell fate, although a clear explanation of their behav-iour has not...

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