... with 5% skim milkand then probed with the appropriate primary antibody(claudin-3 antibody; Abcam, Cambridge, MA, USA;SLC2 5A4 mAb, Abnova, Taipei, Taiwan; HLA Class 1 A1 antibody, Abcam; Tapasin ... Healthcare(Central Plaza, Singapore). Ammonium persulfate andN,N,N¢,N¢-tetramethylenediamine were purchased from Amersham Pharmacia (Piscataway, NJ, USA). EDTA waspurchased from Merck (Darmstadt, Germany). ... claudin-3; CLDN4, cluadin-4; CRC, colorectalcarcinoma; HLA, human leukocyte antigen; HLA -A1 , HLA class I histocompatibility antigen A- 1; iTRAQ, isobaric tags with related andabsolute quantitation;...