... without compound (control).
Figure 4 Antiviral effect of chloroform extract of Solanum
nigrum against HCV NS3 protease. Huh-7 cells were transfected
with 0.5 μg of constructed HCV NS3 protease vector ... Anti-HCV activity of methanol extract of Solanum
nigrum. Huh-7 cells were incubated with HCV serum and 100 μg/μl
concentration of Solanum nigrum seeds extract for...
... and C- termini of HCV NS5A was
amplified using sense and anti-sense overlapping primers
(S/7529/GFP- 5’-GCCTCCTCTATGCCCCCCATGGT-
GAGC AAGGGCGAG-3’ and (AS/7547-7564/GFP 5’-
TCCAGGCTCCCCCTCGAGCTTGTACA
GCTCGTCCAT-3’). ... gene (sense 5’-ATCGAATT-
CATCGTGGCTGGCCA-3’ ;anti-sense5’ -CTA-
GAATTCGGCGCGAGCCCCTG-3’ ;probe5’-
GCTTGGTGGTCGAATGGGCAG GTAGCCGGA-3’.
Infectivity assay
An infectivity assay...
... this study.
Author details
1
State Key Laboratory of Crop Biology, College of Agronomy, Shandong
Agricultural University, Tai’an, Shandong 271018, China.
2
Oxford University
Clinical Research Unit, ... Unit, Ho Chi Minh City, Vietnam.
3
MRC Centre for Genomics
and Global Health, University of Oxford, Oxford, UK.
4
Current Address:
Department of Ecology and Evolutionary Biology, Univer...
... Nurse, Cancer Research UK
Your research papers will be:
available free of charge to the entire biomedical community
peer reviewed and published immediately upon acceptance
cited in PubMed and archived ... interests.
Authors' contributions
SR conceived of the study, participated in its design and
coordination and gave a critical view of manuscript writ-
ing. MI collected epidemiolog...
... Ethics Office (formerly Health Sciences Ani-
mal Policy and Welfare Committee) and was conducted
with humane care of the animals in accordance with
guidelines established by the Canadian Council of ... at the University of Alberta,
provided technical assistance with the atomic absorption spectroscopy. The
Cell Imaging Facility of the Department of Oncology at the University of
Alb...
... -AAAGTCGGAGTCAACG-
GATTTG-3’
reverse 5’ -TGTAAACCATGTAGTTCAGATC-
GATGA-3’
Type II Coll: forward 5’ -CGGCTGCACAAAACA-
CACTGC-3’
reverse 5’-CCTTCCGCCCTGCAGAT-3’
Type I Coll: forward 5’ - CAGCCGCTTCACCTA-
CAGC-3’
reverse ... of 12
RESEARC H ARTIC L E Open Access
Anti-inflammatory activity of
monogalactosyldiacylglycerol in human articular
cartilage in vitro: activation of an anti-
inflam...
... MB,
Brown PO, Botstein D, Futcher B: Comprehensive identification
of cell cycle-regulated genes of the yeast Saccharomyces cer-
evisiae by microarray hybridization. Mol Biol Cell 1998,
9:3273-3297.
35. ... efficiency (C1 , C2 ) of each transcription factor
0
T
T
Shifted time
Conversion efficiency
Generate the combinatorial expression profile of the two regulators for each combinatio...
... specific in this respect.
Sensitivities ranged from 64% for assay of Abs against
teichoic acid to 72% for assays of antibody to
peptidoglycan and α-toxin. The specificity of assays for
antibody ... to cell wall peptidoglycan of Staphylococcus aureus in
patients with serious staphylococcal infections. J Infect Dis 1981,
144(1):1-9.
31. Martin RR, Greenberg SB, Wallace RJ. Stap...