... g and c 14-3-3 isoforms, and peptides that bind to the 14-3-3 binding pocket prevent this interaction. The region containingSer218 in this CKIa binding site was mutated and the interaction between CKIa ... after the incu-bation of the PPase with the inhibitor, vanadate (VO4)(lanes 4) indicated that the interaction is phospho-dependent and that the effect was caused by PPasemasking the binding ... nonphosphorylated syn-thetic peptide corresponding to this region could binda number of proteins from the brain, including actin,importin-a1, importin-b, protein phosphatase 2Ac,centaurin-a1 and HMG1 [25]....