báo cáo khoa học: " BYPASS1: synthesis of the mobile root-derived signal requires active root growth and arrests early leaf development" ppsx
báo cáo khoa học: " BYPASS1: synthesis of the mobile root-derived signal requires active root growth and arrests early leaf development" ppsx
... 11:28http://www.biomedcentral.com/1471-2229/11/28Page 8 of 10RESEARCH ARTICLE Open Access BYPASS1: synthesis of the mobile root- derived signal requires active root growth and arrests early leaf developmentJaimie M Van ... 147:595-610.doi:10.1186/1471-2229-11-28Cite this article as: Van Norman et al.: BYPASS1: synthesis of the mobile root- derived signal requires active root growth and arrests early leaf development. BMC Plant Biology 2011 11:28.Submit ... bps1 roots (including the pri-mary) expand radially, and this radial growth might alsosustain synthesis of the bps1 signal. bps1 signal transmissionMovement of the bps1 signal from the root...
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Báo cáo khoa học: "Dosimetric consequences of the shift towards computed tomography guided target definition and planning for breast conserving radiotherapy" pdf
... (2D) reconstruction of all surgical clips, and the marked location of the scar. On the basis of the posi-tion of the clips and the available pre-operative informa-tion, the assumed lumpectomy ... significant increase of the dose delivered to the lungs. In these cases, inadequate cov-erage of the medial and lateral aspects of the breast PTV isaccepted as long as adequate coverage of the boost PTV ... performeddose-planning and dose-calculation, performed the datacollection and analysis and drafted the manuscript. WVDparticipated in the design of the study, performed the def-inition of the conventional...
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Báo cáo khoa học: Heterologous synthesis of cytochrome c¢ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery ppt
... as c¢ and c552, respectively. The arrow and arrowhead indicate the positions of the PHCP and PH c552proteins,respectively. The band denoted by the asterisk on the right-handside is the result ... used for the maintenance and propagation of all plasmids. The E. coli RI89, RI242 and JCB387 strains were examined with regard to the synthesis of exogenous PHCP and PH c552proteins. The RI89 ... synthesize the PHCP and PH c552proteins,confirming that the observed difference between the two proteins in the RI242 strain is a result of the absence of the DsbD protein.PHCP was also synthesized...
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Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot
... Amilestone in the study of peptide s ynthetases was the determination of the A-domain structure of GrsAPhe-A [8].Aspects of speci®city and binding were superimposed onother A-domains and with the help ... condensation [5] and the corresponding residue of D151 in the SrfB2Asp-CATsurfactin synthetase essential for the synthesis of surfactinin B. subtilis, shown in vivo [21,30]. In the present study ... tointerference with other reactive residues in NRPS moduleslike the thiol g roup of the 4 ¢-phosphopantethein-moiety or the aminoacyladenylate of the activated amino acid. Fur-thermore, N-a-tosyl-L-phenylalanylchloromethane...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... bind each other’s metals, they are inactivewhen the wrong metal is incorporated at the active site[3]. However, several SODs are active in the presence of either Fe or Mn. These types of SODs ... ligands in the Mn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed anequatorial plane (Fig. 3B). The metal ... metal cofactor.Figure 3D shows the superimposition of the active site structures of apo, Mn-bound and Fe-bound Ape-SODs. The most significant difference among themwas related to the OH of Tyr39,...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... binding and radical transfer from the tyrosyl rad-ical (Y122) of the R2 subunit to the cysteine (C439)found in the active site of the R1 subunit; (b) the abstraction of two protons and water ... this RNR and that of other class 1RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E.We consider the first option unlikely. Under these ... to the catalytic site of the R1E subunit. Asreported in the Results, upon completion of substrateconversion, the radical is then rapidly passed backfrom the R1E subunit to the tyrosyl of the...
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Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf
... monomer and dimer, and that Thr binding shifts the equilibrium to dimer formation. In the absence of Thr, an outwardshift of b-strands near the Thr-binding site (site 1) and a concomitant loss of the ... regulatory mechanism of Thr and the struc-tural features responsible for the high thermostability of TtAKb.Results and DiscussionModel quality The crystal structure of the Thr-bound form of TtAKb(TtAKb-Thr) ... chains A and B)sections of the structure are not seen on the map,probably owing to disorder of these regions. The over-all geometry of the model according to the procheckprogram [21] is of good...
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Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... that the activation of IRF-3 by M-pIC treatment is mediatedby the TLR3 ⁄ TRIF signaling pathway. We obtainedfurther evidence by examining the status of the phos-phorylation and dimerization of ... PH5CH8 cells. First, weconfirmed the effect of NS3-4A on the activation of the IFN-b gene promoter by the Cardif exogenouslyexpressed in PH5CH8 cells. The results of the lucif-erase reporter assay ... examined the effects of NS3-4A on the phosphorylation and dimerization of IRF-3 inPH5CH8 cells. We observed that both T-pIC and M-pIC treatments induced the phosphorylation atSer386 and Ser396 of...
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... between the twosteps of the TES reaction The substrate guanosine to be excised (G1) and its2¢-OH group are required for the second step of TES [48], similar to (if not the same as) the role of the ... the integrity of the bound species during gel electrophoresis, the gel and the running buffer were made of H10Mg buffer and were prewarmed to 44 °C before the samples wereloaded. The bound and ... that there is room for improve-ment in terms of the rate of reaction. This would bebeneficial not so much in terms of the rate of the over-all reaction, as the cleavage reaction is not the limitingstep...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... according to the residue in the mid-point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase. The observed ... planes of the bonds between Trp215–Gly216 and Cys191–Gln192sandwich the phenyl ring of benzamidine. The DESC1 S1 pocket resembles the thrombin S1 pockettype because of the presence of an Ala rather ... Lys224–Tyr228 (the back of the pocket) and the disulfide bridgeCys191–Cys220 (the front of the pocket) (Fig. 4A). The backbones of these segments form a deep hydrophobicpocket with the negatively...
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