báo cáo khoa học: "Characterization of a caleosin expressed during olive (Olea europaea L ) pollen ontogeny" pdf

báo cáo khoa học: "Characterization of a caleosin expressed during olive (Olea europaea L.) pollen ontogeny" pdf

... of caleosin in the olive pollen and locular fluid. Sections from olive anthers atthe mid microspore (A) , young pollen grain (B), mid bicellular pollen grain (C) and mature pollen grain (D) stages ... from olive anthers at the pollen mother cell (PMC), tetrad (Te),microspore (Mi), young pollen grain (YP), mid bicellular pollen grain(MBP) and mature pollen grain (MP) stages. (B) Western blot as ... in olive (Olea europaea) andavocado (Persea americana). Plant Sci 1993, 93:203-210.26. Alché JD, Castro AJ, Rodríguez-Garc a MI: Expression of oleosin genes inthe olive (Olea europaea L. ) anther....

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... for absorption by thecell, whereas intracellular peptidases play a major rolein regulation of metabolism [2].The families of chymo(trypsin) (S 1) and subtilisin (S 8) are regarded as the largest ... peptidases areclearly indicated by the fact that 2% of all genesencode peptidases (or their homologues) in all kinds of organisms [1]. Extracellular peptidases hydrolyse largeproteins into smaller ... NcoI was from New Eng-land Biolabs (Beverly, MA, USA). Escherichia coli TOP10[F- mcrA n(mrr-hsdRMS-mcrBC) u80lacZnM15 nlacX74deoR recA1 araD139 n(araAleu)7697 galU galK rpsLendA1 nupG] and...

Báo cáo khoa học: Characterization of a functionally expressed dipeptidyl aminopeptidase III from Drosophila melanogaster doc

... Western blot analysis of overexpressedD. melanogaster DPP III. (A) D. melanogaster DPP III partiallypuriﬁed from cytosol of transfected S2cells (1 lg) was electrophoresedon a 7.5% (w/v) acrylamide ... Polyvar)and image analysis software (Spot RT, Diagnostic Instru-ments, USA).Partial puriﬁcation of a putativeD. melanogasterDPP III expressed in S2cellsTypically, 50 mL of cell culture were ... S2cells.Cytosolic samples were prepared from transfected S2cells afterinduction and electrophoresed. (A) Cytosolic sample (30 lgproteinper lane) was separated on a 7.5% (w/v) acrylamide gel and stainedwith...

Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf

... Sallantin, M., Huet, J C., Demarteau, C. & Pernollet, J C. (199 0) Reassessment of commercially available molecular weight stan-dards for peptide sodium dodecyl sulfate-polyacrylamidegel ... Angeli, S., Marchese, S., Brandazza, A. ,Ferrara, L. , Pelosi, P. & Scaloni, A. (200 1) Bacterial expressionand conformational analysis of a chemosensory protein fromSchistocerca gregaria. ... organs of Eurycantha calcarata (In-sects, Phasmatodea). Insect Biochem. Mol. Biol. 30, 1091–1098.28. Nomura ,A. ,Kawasaki,K.,Kubo,T.&Natori,S.(1992)Puri-ﬁcation and localization of p10, a...

Báo cáo khoa học: Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae potx

... kDa), aldolase (158 kDa), conalbumin (75 kDa) andalbumin (43 kDa) (GE Healthcare HMW Gel FiltrationStandards; GE Healthcare, Milwaukee, WI, USA). In addi-tion, P. putida BFDC was included as ... tryptophancatabolism [2]. It may also be involved in tyrosinemetabolism but, when alternative pathways are avail-able, it is unlikely to play any signiﬁcant role in thecatabolism of the branched-chain ... E54 5L vari-ant show allosteric activation? Again, the answer maylie with AbPPDC. It has been proposed that, ratherthan the typical Glu-Asp-His motif, AbPPDC has anAsp-Asp-His catalytic triad...

Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot

... CLAP_1:AAGATCTTCTGAATGTGATTATATGCGGCTGTGTTTTCTAATAGATTTCTAGATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1888 CLAP_2:AAGATCTTCTGAATGTGATTATATGCGGCTGTGTTTTCTAATAGATTTCTAGATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (A) 48 ... CLAP_1:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTAAAATTAGAAAAATTGAAAGACAAGCTAATGAAAGCTTATTGCCGATTGGAAAGT 1300 CLAP_2:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTAAAATTAGAAAAATTGAAAGACAAGCTAATGAAAGCTTATTGCCGATTGGAAAGT 1232CLAP_1:TTGCTTGGGGGGAAGACTCGTTACAATTCTTTTTCTTTATTTTCTTTTTAGGTAGCTTCTTTATTTTATTTTTTT -A TCTCTTTCTTGATTTTCTTTTCT ... 121CLAP_1:GACATGAAACTGAGAACTCTCCTCACAAAGAGGGACTTGAGGATTAATTTGTATGACAATGGGCAGACAATTCTTGCCGGTGGGAAACGTATAAATGGAT 500 CLAP_2:GACATGAAACTGAGAACTCTCCTCACAAAGAGGGACTTGAGGATTAATTTGTATGACAATGGGCAGACAATTCTTGCCGGTGGGAAACGTATAAATGGAT...

Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt

... differentially expressed. One of the polypeptides migrated with an apparent molecularmass of 14.4 kDa and a pI value of  5.8 (Fig. 1,spot 1 5) and was only found in cells cultured at high(0.8 lm) ... Methylococcus capsulatus(Bath)Odd A. Karlsen, Linda Ramsevik, Live J. Bruseth, Øivind Larsen*, Annette Brenner,Frode S. Berven, Harald B. Jensen and Johan R. LillehaugDepartment of Molecular Biology, ... multiple alignmentsand the crystallized myohaemerythrin from Themistezostericola as a template (Fig. 8A) [11]. Hence, compu-tational analysis modelled the putative haemerythrin to a four a- helix...

Báo cáo khoa học: Characterization of a high-afﬁnity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt

... the available databases, itwas recently established by NMR studies and molecularmodeling that PA1b belongs to the cystine-knot family [8].Characterized only recently as a structural family ... themicrosomal fraction of S. oryzae extract was found to besaturable and reversible, with an a nity (Kd )of2 .6nM,and a high maximal binding capacity (Bmax )of4 0pmolÆmg )1 of protein. A binding ... areexpensive and not always applicable. Plant protection alsotakes advantage of the genetic resources residing in cropplants to create resistant varieties, and of the use of biological toxins to build...

Báo cáo khoa học: Characterization of a membrane-bound aminopeptidase puriﬁed from Acyrthosiphon pisum midgut cells A major binding site for toxic mannose lectins pptx

... substrate concentrations. SEM values were calculated by ﬁtting data by a weighted linear regression usingthe software SigmaPlotÒ. AlabNA, L- alanine-b-naphthylamide; AlapNA, L- alanine-p-nitroanilide; ... molitor [22], soluble Tineola bisselliela (Lepido-ptera) aminopeptidase [35,36], soluble Attagenusmegatoma (Coleoptera) aminopeptidase [37], solubleand microvillar R. americana (Diptera) aminopeptidas-es ... [17] and APN4 (BmAPN 4) (GenBank accession numberBAA3371 5); Epiphyas postvittana, Epo, APN(EpAPN) (GenBank accession number AAF9970 1) [13]; Lymantria dispar, Ldi, APN1 (LdAPN 1) and APN2 (LdAPN2)...

Báo cáo khoa học: Characterization of a novel long-chain acyl-CoA thioesterase from Alcaligenes faecalis docx

... theabnormal accumulation of intracellular acyl-CoA.We have isolated a thioesterase from Alcaligenesfaecalis ISH108 and demonstrated its application inchemoselective and racemization free deacylation ... 3B.Intracellular localization of thioesteraseWe carried out electron microscopic immunogold labe-ling studies with ultrathin sections of Alcaligenes cellsto localize thioesterase at the ultrastructural ... 2381Characterization of a novel long-chain acyl-CoAthioesterase from Alcaligenes faecalisPuja Shahi*†, Ish Kumar*‡, Ritu Sharma, Shefali Sanger and Ravinder S. JollyInstitute of Microbial Technology,...

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