... Forward primerAcs 5¢-TAATACGACTCACTATAGGGA*5¢-AACACACCATTCCTGCCAAC-3¢CCACCACAGGTCGCGCC-3¢AcnB 5¢-TAATACGACTCACTATAGGGA*5¢-CTCACACGCTGCTGATGTTC-3¢CGTGGTTACGCACTTCACC-3¢PrpD 5¢-TAATACGACTCACTATAGGGA*5¢-AACATCGGCGCGATGATCC-3¢TCGCTGCTTCAACTGCCG-3PrpE ... hydroxy or unsaturated dicarboxylic and tricarboxylic acids such as trans-aconitate, threo-2-methyl-isocitrate and erythro-2-methylisocitrate,D-malate and L-malate, and (R)-citramalate and ... trans-aconitate,D-malate and L-malate, fumarate, maleate,D-tartrate and meso-tartrate,D-citramalate and L-citramalate,mesaconate, citraconate, itaconate, and (R,S)-3-methylitaconate.SubstrateConcentration(mM)ActivityUÆmg)1%(2S,3S)-Methylcitrate...