Báo cáo khoa hoc:" Vitamin D and oestrogen receptor polymorphisms in developmental dysplasia of the hip and primary protrusio acetabuli – A preliminary study" ppt

... Hospital of NorthStaffordshire by two individuals (BK, CD), and the resultsvalidated by an independent, blinded observer examining the agarose gels (AF). 10% of the assays were repeated and analysed ... severity of the conditions.Patients and methodsWe recruited 45 patient with DDH and 20 patients withPPA. In all patients, a diagnosis of DDH or PPA was madeon the basis of clinical and radiographic ... Dysplasia of the hip (DDH) and Primary Protrusio Acetabuli (PPA) encompass the spectrum of acetabular development from a shallow acetabulum in DDH to a deep acetabulum in PPA. Both have, as yet, anindeterminate...

Báo cáo khoa học: Vitamin D stimulates apoptosis in gastric cancer cells in synergy with trichostatin A ⁄sodium butyrate-induced and 5-aza-2¢-deoxycytidine-induced PTEN upregulation ppt

... histone deacetylase (HDAC) inhibitors trichosta-tin A (TSA) and sodium butyrate (NaBu), and the methylation inhibitor 5-aza-2¢-deoxycytidine (5-Aza).Fig. S2. Vitamin D induced apoptosis in gastric ... revealed that VD3 could induce differentia-tion and cell cycle arrest in a number of malignantmelanoma cells, including those in myeloid leukemia, and breast, prostate, colon, skin and brain cancer ... 5-Aza (Fig. 6D) . Thus, we concluded that PTEN partici-pated in vitamin D- induced apoptosis, and epigeneticmodiﬁcations such as acetylation and DNA methylationwere involved in the regulation...

Báo cáo khoa hoc:" Vitamin D receptor gene polymorphisms in multiple sclerosis patients in northwest Greece" docx

... polymorphisms of the VDR gene may modify vitamin D function and metabolism and have been exam-ined in studies evaluating the role of vitamin D on MS [9].Several such studies that have investigated the ... cited.responsible for the BMD determination using DXA in the laboratory of nuclear medicine and overview of the study. All authors read and approved the final manuscript.Competing interests The ... and 81 age and sex-matched healthy individuals. Genotyping of Taq-I(rs731236) and Bsm-I (rs1544410) was performed using TaqMan®SNP Genotyping Assay. All patients and controlshad determination...

Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

... in the UDP-Gal. Unless preincubation was performed, an initialburst phase was observed as an artefact. The assay was ini-tiated by adding 3–1 5 nmol of the epimerase. The rate of formation of ... the rectangular denting of the upper domains of each lobe indicates the binding site of 5¢-UMPex vivo; the shaded rectangle indicates 5¢-UMP bound as isolated; the open rectangle indicates added ... (1) and reconstituted (2) epimerase. The horizontal dotted lines indicate initial absorbance of native (1) and reconstituted (2) epimerase. Durations of initial lag of catalysisare indicated by...

Báo cáo khoa học: "Carbonic anhydrase XII expression is associated with histologic grade of cervical cancer and superior radiotherapy outcome" ppsx

... immunohistochemicalstudy.Statistical analysis of the CA12 expression, histologicgrade, and radiotherapy outcomes The primary endpoints for radiotherapy outcome wereDFSandLRFS.LRFSandDFSwerecalculatedasthedate ... tumors was analyzedindividually and then as a new combined parameter todetermine whether the combined parameter might beclinically useful in further individualized prediction of the prognosis of ... used histo logic grading system.Adenocarcinoma was graded by conventional methods,based on the architectural and nuclear features [10].Immunostaining for CA12 was performed by using the avidin-biotin...

Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

... antibody to normalize for loading.Statistical analysisAll values are expressed as mean ± standard deviation.Statistical signiﬁcance was determined using a one-wayHSS and mitochondrial permeability ... is a key step in DNA damage-induced apoptosis. To further understand the protec-tive effect of HSS against CCCP-induced apoptosis, the activation of caspase-3 was examined using the enzymatic ... mgÆmL)1. The mixture was incubated for 10 min in the dark at roomtemperature. The cells were then resuspended in propidiumiodide solution, and incubated again in the dark foranother 30 min at room...

Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt

... thaliana. (A) proBPM1:GUS : hydathodes and stipules of 5-day-old seedlings showed staining (a c), as did fully developed siliques (d; base and stigma region) and vascular tissue of rosette leaves ... of meprins A and B and the TRAF-C domain, and, like the BTB domain, facilitates protein–proteininteraction [24]. Meprins are tissue-speciﬁc metalloen-dopeptidases implicated in developmental ... Rosette leaves werestained in the vascular tissue of the leaf blade, whereas the petiole and older parts of the midrib remained unstained (b). However, the cau-line leaf blade was stained very...

Báo cáo khoa học: Gonadotropin-releasing hormone: GnRH receptor signaling in extrapituitary tissues docx

... both androgen-dependent and androgen-independent prostate cancer cells by modu-lating E-cadherin-mediated cell–cell contacts and pro-duction of uPA and its inhibitor (plasminogenactivator inhibitor-1) ... stimulate pituitary gonadotropes. On the other hand, sustained administration of the peptidebrings about a short initial stimulation that is rapidlyfollowed by a decrease in gonadotropin synthesis ... Volmat V & Lenormand P (2002) Fidel-ity and spatio-temporal control in MAP kinase(ERKs) signalling. Biochem Pharmacol 64, 75 5–7 63.82 Chakraborti S, Mandal M, Das S, Mandal A & Chak-raborti...

báo cáo khoa học: " Using single cell cultivation system for on-chip monitoring of the interdivision timer in Chlamydomonas reinhardtii cell cycle" pps

... phase at the time indicated by the white arrowhead. TLDis the interdivision phase duration under the LD condition, and μLis the volume increase rate during the light period calculated as in ... interdivision phase.Materials and methodsStrain and culture conditionsWe used a central-pair-lacking (non-mo tile mutant)strain of Chlamydomonas reinhardtii,thepf18+strain.We used a minimal ... on the basis of changes in cell size and foundthat, regardless of the amount of photosynthetically active radiation (PAR) and the extent of illumination, the length of the interdivision phase...

báo cáo sinh học:" Intent to migrate among nursing students in Uganda: Measures of the brain drain in the next generation of health professionals" pot

... University of Washington, Seattle, USA, 2Department of Nursing, Aga Khan University School of Nursing, Kampala, Uganda, 3Department of Medicine, Makerere University, Kampala, Uganda and 4Department ... University and Aga Khan Uni-versity, and the staff and students there who assisted us. We appreciate the enthusiastic participation of nursing students in our study. Elijah Ssema-ganda and Beatrice ... and analysis, and wrote the paper. Esther Nderitu and Anneke Zuyderduin assisted with data collection atAga Khan University. Sam Luboga provided faculty guid-ance at Makerere University. Hagopian...

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