... Approximately 0.4 mg of protein was treated with 10 mU of glycosidase F (Sigma-Aldrich Inc.) at 37 °C for 16 h. For crosslinking of proteins, slices of cotyledonswere homogenized with 20 strokes of a Dounce ... Program for Promotion of Basic Research Activities for Innovative Biosciences,and a Grant-in-Aid for Exploratory Research from theMinistry of Education, Culture, Sports, Science andTechnology of ... (B) In vitro translation of GmPDIL-3a. Translation reactions were per-formed without (lane 1) or with (lane 2) 1 lg of wild -type GmPDIL-3a mRNA or mutantGmPDIL-3a mRNA, of which the first (lane3),...