báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps

... Open AccessProfiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparationMohammad ... optimized purification of such proteins and preparation of nanomagnetisable matrices. Results: Marked differences in the performance of nanomagnetic matrices, principally on the basis of their specificbinding ... applied for all nanomagnetic matrices, butprovide a framework which could be used to profiling and quantitative evaluation of other magnetisable matrices and also provide helpful hints for those...

báo cáo khoa học: "Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications" pps

... responsible for the conception of the research and Han, Huang, Liang, Ho and Wang areresponsible for the execution and for data collection;Chang is responsible for initial drafting and revisions of the ... and functional characterizations of these ex vivo prepared immune effector cells are necessaryto facilitate their clinical applications. Here, we refined and compared three of the state -of- the- art ... preparation approaches, the IFN-γ and the CD137selection methods for emergency preparation of therapeu-tic cells, and a DC-immune cell coculture method for the expansion of antigen-specific immune...

báo cáo khoa học:" Biological and biomechanical evaluation of interface reaction at conical screw-type implants" doc

... include the need to achievea primary congruence between the implant and the bonedirectly after insertion, the need to insert the implant withminimal surgical trauma and the capability of the implantsurface ... The gross morphology of the implants was designed with the help of finite element analysis (FEA). The geometry of the implant was designed to allow micromovements of amagnitude between 500 and ... perpen-dicular to the implant, newly synthesized collagen in the vicinity of the surface appeared to form a felt-like matrix parallel to the surface. Intimate bone contact was pre-sented at the neck of implants....

Báo cáo khoa học: FLIP and MAPK play crucial roles in the MLN51-mediated hyperproliferation of ﬁbroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis pdf

... wasassessed in the presence or absence of 10 lgÆmL)1 of a-amanitin for 20 h (A),25 lgÆmL)1 of cycloheximide (CHX) for 20 h(B) and 20 lM MG-132 for 6 h (C). The expression of MLN51 was assessed ... wasassessed in the presence or absence of 20 lM MG-132 for 6 h. The expression of MLN51 was assessed by WB analysis (left). The histogram (right) represents the relativeband intensity of the WB data, ... proportion of FLSs, because of their constitutive expression of the FLICE-inhibitory protein (FLIP) [11]. FLIP expressionmediates the recruitment and activation of nuclear fac-tor-jB kinase and several...

Báo cáo khoa học: "Fast and Scalable Decoding with Language Model Look-Ahead for Phrase-based Statistical Machine Translation" potx

... Just be-fore the loop we can therefore traverse the trie once for the current history and each of its lower order n-grams and store the pointers to the resulting nodes.To retrieve the LM look-ahead ... track of the number of hypothesis expansions and LM computations. The LM score pre-sorting affects both the set of phrasecandidates due to observation histogram pruning and the order in which they ... pre-computed estimate of the full LM costs for each phrase. We detail the implementation of these methods and analyze theireffect with respect to the number of LM computa-tions and hypothesis expansions...

Báo cáo khoa học: "Pancreatic and multiorgan resection with inferior vena cava reconstruction for retroperitoneal leiomyosarcoma" doc

... resistant to chemo-therapy and radiotherapy, and complete resection of the tumor is the only known method for a chance of cure. The prognosis for leiomyosarcoma of the IVC treated medi-cally ... confirmed the preoperative findings, and no othermetastases were present. The tumor appeared to arisefrom the IVC at the level of the left renal vein and extendintraluminally in the IVC up to the ... adrenal gland, spleen, and distal pancreas. b, Leiomyosar-coma is seen in the lumen of the vena cava. The vena cava wall is on the right. c, Tumor fills the bottom of the picture pushing into the...

báo cáo khoa học: "Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells" doc

... of Biochemistry, Faculty of Medicine, Chiang Mai University,Chiang Mai 50200, ThailandFull list of author information is available at the end of the articleBanjerdpongchai et al. Journal of ... -dichlorofluores-cein diacetate (for ROS detection) were added for 15 min at 37°C and the cells are then subjected to flowcytometry. For flow cyt omet ric assessment of DNA fragmentation and cell ... determine the existence of cell cyclearrest.Assay of caspase-3 and caspase-8 activityCleavage of the fluorogenic peptide substrates DEVD-AMC and IETD-AMC, indicative of caspase-3-like and caspase-8-like...

báo cáo khoa học: " Specifying and reporting complex behaviour change interventions: the need for a scientific method" pot

... illness, and in health professionals and othersresponsible for delivering effective, evidence-based publichealth and healthcare. In the field of implementationresearch, thousands of studies ... across the United States, many of the replication sites delivered serv-ices in their offices, not family homes and, predictably, the outcomes were disappointing [26]. The philosophy and values of ... measured the presence or strength of the interven-tion in practice, and fewer still included such measures in the analyses of the results. Thus, we are often left knowingvery little about the details...

Báo cáo khoa học: Copper-containing nitrite reductase fromPseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center pot

... and Shain, and plotted vs. the inverse of the scan rate. The plotsyielded straight lines, conﬁrming the applicability of the Nicholson and Shain theory to the present system, and the variation of the ... favoring the electron-transfer process. Analysis of the EPR spectrum of the turnover form of the enzyme also suggests that the electron-transfer process between T1Cu and T2Cu is the fastest of the three ... CoCl2,0.6andNa2B2O7,0.1. The optical density, the concentrations of nitrate [17] and nitrite [18], the pO2 and the pH of the medium weremonitored, and the composition of the gaseous...

Báo cáo khoa học: A pathway through interferon-c is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cells pot

... Strongsuppression of the LPS-induced production of NO and IFN-c indicates that the signals of LPS for the produc-tion of these mediators are transduced through CD14.Stimulatory effect of IFN-c and IL-12 ... response of the HeJ culture to LPSwas observed for the production of NO, IFN-c and IL-12p70 (data not shown).We also examined the effect of IL-12 and IL-18 on NOproduction and found that the combined ... (TLR4)–MD-2 complex [8] for the production of mediators, including NO. Production of NO is governed by the activity of three NO synthase(NOS) isoforms in which neuronal NOS and endothelialNOS are constitutively...

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