... Open Access
The anti-myeloma activity of a novel purine
scaffold HSP90 inhibitor PU-H71 is via inhibition
of both HSP9 0A and HSP90B1
Saad Z Usmani
1*
, Robert D Bona
2
, Gabriela Chiosis
3
, Zihai ... cytosolic HSP90 (HSP90AA1), the ER
paralogue gp96 (HSP90B1) and the mitochondrial member TRAP1 (HSP90L). We evaluated the in vitro anti-tumor
activity an...
... protein
and its truncation products. K
+
activated p-nitrophenyl-
phosphatase as a partial activity of Na
+
/K
+
ATPase was
measured as described previously [17]. S tatistical analysis of
the comparison ... plasma membranes of mammalian cells. The enzyme
is a heterodimer of a 100 kDa catalytic subunit and a
heavily glycosylated b subunit of about 55 kDa [1–3].
Ouabai...
... also for the
antimicrobial activity of these peptides. In this work, we
have taken advantage of the fact that bacteria of the class
Mollicutes are devoid of an outer membrane and of a cell
wall, ... of membrane
depolarization and the delay necessary to reach a steady-
state level of depolarization. Actually, 0.1 l
M
melittin
totally depolarized the A. laidlawii membra...
... this interaction has an
effect on the enzyme activity of the kinase. In this
study, we demonstrate that both kinases (SRPK 1a and
SRPK1) interact with both scaffold attachment factors
(SAFB1 and ... protein-specific kinase involved in mediat-
ing the interaction and localization of pre-mRNA splic-
ing factors in mammalian cells. J Cell Biol 140, 737–750.
9 Nakagawa O, Arnold...
... divided by a factor of 33. UV spectra were acquired
using a Cary 5G UV-Vis-NIR spectrophotometer (Varian
Canada, Mississauga, Canada) in a 1 cm quartz cuvette at
room temperature (22 °C) and recorded ... ESI-TOF mass spectrometer (Waters Micromass,
Mississauga, Canada) at room temperature (22 °C), and
recorded using the mass lynx software package version
4.0. The ESI-TOF spectrome...
... 5'-CACCGAGAAGTCAA-
CAGCTCGGTCATCGAC-3' and 5'-TCAAGTCTTC-
CAATTTCTACCCAGTGCTGC-3' for AtYLMG1-1, 5'-
CCTCAACATATATAACACCATC-3' and 5'-GACAG-
GTTCAGGTCATAGAAG-3' ... 5'-GACAG-
GTTCAGGTCATAGAAG-3' for At5g21920, 5'-TATCT-
GAACACTCCGTTGACGGTA-3' and 5'-CAAAGATA
AACGGAATACGATC-3' for At4g27990, 5'-GCAATGG-
GAAGCAGTGG...
... inter-
actions are mediated through many salt bridges and
van der Waals contacts between a
2
and b
4
of RRM 1
and b
2
and a
1
of RRM 2, respectively (Fig. 6D).
Another interesting example of RRM–RRM ... of another protein that acts as a cofactor.
Both U2B¢ and CBP20 need a cofactor, U 2A and
CBP80, respectively, to recognize RNA. Ternary
structures of these com...
... fractions was measured and the
purity of pyridoxal kinase was determined by SDS ⁄ PAGE
[29] and MALDI-TOF-MS (see Supplementary material).
General analytical methods
Enzymatic formation of vitamin ... unphosphorylated dietary precursors catalysed by
PKH is required ubiquitously in mammalian tissues
[15]. Decreased activity of PKH leads inter alia to a
decreased availability o...
... exclusively of archaeal RadA protein, a homolog of
bacterial RecA, eukaryotic Rad51 and archaeal RadB. This protein is
involved in DNA repair and recombination
[42,188]
PCNA PCNA, or cyclin, is a non-histone ... Dmc1
enhances DNA topoisomerase II catenation activity
independent of sequence homology. Chromosoma 117,
297–302.
163 Koshiyama A, Hamada FN, Namekawa SH, Iwabata...
...
(Shaw
and
Allard,
198 2a)
and
natural
populations
(Neale
and
Adams,
1985).
Apart
from
the
theoretical
value
of
such
studies
they
are
also
of
prac-
tical
interest,
especially ...
natural
populations
(Neale
and
Adams,
1985;
El-Kassaby
et
al,
1987;
Muona
and
Harju,
1989)
and
has
been
explained
mainly
by
the
family
struc-
ture
of
a
stand.
I...