... days in the differenti-ation medium: DMEM containing 10% fetal bovine serumand IDM (10 lgÆmL)1insulin, 1 lm dexamethasone, and0.5 mm IDM). Cells were then maintained in DMEM con-taining ... 3T3-L1 cells were maintained in a hypoxia chamber (Invivo 400; Ruskinn Inc., Cincinnati,OH, USA) constantly maintained at 1% O2. Culturemedium was replaced every other day inside the chamber.For ... regulator of energy metabolism in the fat body of fruit flies [3]. In vertebrates, miR-375 and miR-376, both of which are abundantlyexpressed in pancreatic b-cells, are involved in the con-trol of insulin...