... nine autoclaved 1 . 8- mL microcentrifuge tubes as follows: TG 1-1 0–1, TG 1- 10–2, TG 1-1 0–3, TG 1-1 0–4, CJ23 6-1 0–4, CJ23 6-1 0–5, CJ23 6-1 0–6, CJ236–10–7, and CJ23 6-1 0 8 . Into each, pipet ... M Tris-HCl, pH 7.4, to neutralize the pH and mix gently.Isolation of Single-Chain Antibodies 239269From: Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited ... immunohistochemistry and FACS (see Note 10).242 Radoˇsevi´c and van Ewijk255From: Methods in Molecular Biology, vol. 1 78: Antibody Phage Display: Methods and Protocols Edited by: P. M. O’Brien and R. Aitken...