... 22Electroporation, methods, 52, 66, 94, 2 93, 30 9, 31 4, 35 2, 36 3, 36 5preparation of cells, 51, 99, 2 93, 30 9, 35 2, 35 4, 36 3ELISA,antigen capture, 115, 195, 198 8. pcDNA3 vector (Invitrogen, Leek, The Netherlands). ... 31 9CD28, 31 9CD36, 15CD40, 121CD55, 17cDNA,storage, 55synthesis, 47, 65, 75, 83, 90, 97, 117,126, 211c-erbB-2, 39 Chromatography,immobilized metal affinity (IMAC), 32 6, 32 8, 33 4, 33 7 33 9, 34 6, 35 3, ... 39 Chromatography,immobilized metal affinity (IMAC), 32 6, 32 8, 33 4, 33 7 33 9, 34 6, 35 3, 35 4, 35 6ion exchange, 32 6, 32 8, 33 7size exclusion, 32 7, 33 8, 33 9, 34 6Colorectal cancer, 227Complement factors, 182Competent...