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Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites potx

Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites potx

Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites potx

... addressed the question of whether secreted proteins of parasitic nematodes show accel-erated evolution, and our results indicate that this is the case. The predominance of predicted secreted proteins ... of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted pro-teins in parasites& lt;p>Parasitism is a highly successful mode of life and one that ... encodinghomologs of Ancylostoma secreted protein [2], five clusters of C-type and S-type lectins [55] and seven clusters forcysteine proteinases [56]. Proteins bearing signal sequences are less evolutionarily...
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báo cáo khoa học:

báo cáo khoa học: " Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress" pot

... 9% unigenes) and Populustrichocarpa (212, 9% unigenes). The availability of the whole genome and predicted proteins of these species and limited sequence information of legumes in the databas ... i n 40%-60% of the total chickpeaproduction [4]. Terminal drought, which occurs at the pod filling and seed-developing stage of the crop and incr eases in severity at the end of the season, ... and HO062183)] and also these genes sho wn to be involved in drought response in several other plants [71,72 ].Upregulation of genes involved in ion binding and trans-port activities [e.g., ATP-binding proteins...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... simultaneouslyinteracts with the main phosphate of the substrate [32]. The closer positioning of the N-terminus to the mainprotein body is essential for the activity and, together with the 87–104 ... 23 diminished the midpoint of the thermal denaturation curve (Tm)by7°Candthefreeenergy of unfolding (DGU)by11kJÆmol)1. These values are in very close agreement with those obtained using ... [35S]methionine into newly synthesized proteins after 96 h of incubation with an increasingconcentration of either (Met1)-ONC (M23L) or the activa-ted Pyr-ONC (M23L). The results are shown in Fig....
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Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf

Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf

... betweenb-strand B6 in the substrate-binding domain and strand E2 in the hinge domain, two residues appear toact, at least partly, as structural determinants for the closed conformation of the substrate ... unfolding of the L537W ⁄ E542K and L537W ⁄ E542R mutants in one single heatingcycle. The spectra of the native proteins L537W ⁄ E542K (solid line) and L537W ⁄ E542R (dashed line), as well as the ... ‘head’domain (Fig. 8). In the thermostable L537W ⁄ E542Kdouble mutant, differences in the backbone position of the exposed head domain of up to 4.3 A˚, and of exposed parts of the Rossmann domain of...
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Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

... obtain an insight into the mechanism of thermosta-bility of this protein, we compared sequences of thermo-philic Bt-Lon with those of mesophilic Bs-Lon. The G+Ccontent of the protein-coding ... Effects of temperatureon the DNA-binding activity of Bt-Lon. 25 lL of the solution con-taining 4 lg Bt-Lon and 500 ng plasmid DNA was incubated for20 min at the indicated temperatures and then ... bymonitoring the kinetics of aggregation by light scattering.As shown in Fig. 7, curve 1, denatured insulin formedaggregates in the absence of Bt-Lon. In contrast, at the 3 : 1(w/w) ratio of insulin...
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Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx

Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx

... Itconsists of the intrinsic subunits CP47, C P43, D2 and D1, and the extrinsic proteins of the oxyge n-evolving complex the 33 k Da, cyt c550 and 12 k Da. The presence of the cyt c550 in the PSII ... independent on the presence of other extrinsic proteins [44], whereas effective binding of red algal cyt c550to the red algal PSII requires the presence of all of the other extrinsic proteins ... higherplants and green algae contain the 23 kDa (PsbP) and 16 kDa (PsbQ) e xtrinsic proteins. In cyanobacteria and redalgae, these proteins are missing and they a re replaced by the cyt c550 and 12...
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Báo cáo Y học: The Fe-only nitrogenase and the Mo nitrogenase from Rhodobacter capsulatus A comparative study on the redox properties of the metal clusters present in the dinitrogenase components doc

Báo cáo Y học: The Fe-only nitrogenase and the Mo nitrogenase from Rhodobacter capsulatus A comparative study on the redox properties of the metal clusters present in the dinitrogenase components doc

... 1/2 signal in the g ¼ 2regionFig. 1. P-cluster EPR signals of the MoFe protein compared to the EPRsignals detected with the oxidized FeFe protein. TheMoFeproteinsample contained 2 1 mg protein ... the MoFe proteins of otherbacteria. These include: demonstration o f the classicalFig. 6. EPR signals o f the FeFe protein and the MoFe protein underturnover conditions. (A) EPR sp ectra of ... notethat the reducing agent was not entirely removed from FeFeprotein preparations in view of the lability of the proteineven in the presence of only trace amounts of oxygen [8].For the sake of...
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báo cáo hóa học:

báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

... neovascu-larization of hES-ECs and hES-MCs could result in the increase in CBF and the marked promotion of vasculardensity in the ischemic striatum after the transplantation of hES-ECs+MCs. In the hES-EC+MC-injected ... used the term 'eEC' for these ECs in the earlydifferentiation stage. We sorted and expanded these eECs in vitro. These eECs were cultured in the presence of VEGF and 10% FCS and expanded ... VEGF and bFGF in stroke areas [3], and in the present study, the increase of vascular density in saline-injected group on day 28 after MCAo was actuallyobserved. The finding that there was no...
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from dust to stars studies of the formation and early evolution of stars

from dust to stars studies of the formation and early evolution of stars

... reached the main sequence. The term pre-main sequence has been introduced since the first calculations of evolutionary tracks in the HR-diagram in the mid-1950s.2.2 The Quest to Understand the Formation ... raising the issue of the stratification of the interstellar medium (ISM) in the gravitational potential of our Galaxy. Per-haps one of the biggest breakthroughs in the understanding of the ISM ... apicture from a bible print in the 16th century depicting the traditional Judeo-Christian view of the Genesis, the Bible’s version of the creation in whichGod makes the Earth and the Cosmos in six...
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Báo cáo khoa học:

Báo cáo khoa học: "Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods" pdf

... arginine, respectively.Among the 135 streptococci investigated 94 and 4 of the strains fermented inulin and raffinose, respectively.Additionally, 130 of the investigated strains, and the S.uberis ... wasproduced by the majority of the S. uberis strains isolated from clinical cases of bovine mastitis. Comparable to thesefindings most of the S. uberis of the present investigationwere pauA and skc ... wereobserved, indicating that a wide variety of S. uberis strainsmight infect and cause mammary gland infection due to the contamination of the gland from the environment. Thishigh degree of heterogeneity...
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