... betweenb-strand B6 in the substrate-binding domain and strand E2 in the hinge domain, two residues appear toact, at least partly, as structural determinants for the closed conformation of the substrate ... unfolding of the L537W ⁄ E542K and L537W ⁄ E542R mutants in one single heatingcycle. The spectra of the native proteins L537W ⁄ E542K (solid line) and L537W ⁄ E542R (dashed line), as well as the ... ‘head’domain (Fig. 8). In the thermostable L537W ⁄ E542Kdouble mutant, differences in the backbone position of the exposed head domain of up to 4.3 A˚, and of exposed parts of the Rossmann domain of...