... hypophos-phatasia, (c) childhood hypophosphatasia, (d) adulthypophosphatasia and (e) odonto hypophosphatasia[1–4]. Perinatal and infantile forms of hypophosphata-sia are severe and are usually transmitted ... from Qiagen (Hilden, Ger-many); antipain, chymostatin, elastatinal, leupeptin and pepstatin A were obtained from Protein Research Founda-tion (Osaka, Japan); and hygromycin B and (p-amidinophe-nyl) ... chymostatin,elastatinal, leupeptin, pepstatin A) was added to cell lysates and media (10 lg of each protease inhibitor per mL). Thelysates were incubated for 20 min at 37 °C to extractTNSALP....