... glycoprotein oligosaccharides N-linked to asparagine:enzymatic c harac terization of a Galb1,3(4)GlcNAc :a2 ,3-sialyl-transferase and a Gal b1,4GlcNA c :a2 ,6–sialyltransferase from ratliver. J. Biol. ... a MolecularDynamics (STORM) scanner. The bands were quantifiedby the IMAGEQUANTsoftware (Molecular Dynamics,Sunnyvale, CA, USA).Sialyltransferase enzyme assayST6Gal I was assayed as previously ... Invitrogen(Cergy-Pontoise, France). Marathon rapid amplification of cDNA ends (RACE) kit and human leukaemia cellline K562 poly (A) +RNA were from Clontech (PaloAlto,CA,USA).TheDNAgelextractionkitwasfromQiagen...