... Takashi Osaka, Reiko Sakamoto, Akinori Tokunaga,Yuhki Nakatake, Mitsuharu Sato and Nobuaki YoshidaLaboratory of Developmental Genetics, Center for Experimental Medicine and Systems Biology, Institute ... FAC-SCalibur (Becton Dickinson, Franklin Lakes, NJ, USA) and flowjo software (TreeStar, Ashland, OR, USA).Mice and teratoma formationC57BL ⁄ 6J mice and MCH:ICR mice were purchased fromCLEA Japan ... postnatal mouse brain.Mech Dev 101, 217–220.22 Mitsui K, Tokuzawa Y, Itoh H, Segawa K, MurakamiM, Takahashi K, Maruyama M, Maeda M & Yama-naka S (2003) The homeoprotein Nanog is required for maintenance...