Báo cáo khoa học: "Notice of redundant publication: Surgical resection for gastrointestinal stromal tumors (GIST): Experience on 25 patients" docx

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Tài liệu Báo cáo khoa học: Application of a ﬂuorescent cobalamin analogue for analysis of the binding kinetics A study employing recombinant human transcobalamin and intrinsic factor pdf

... proteinconcentrations keeping the initial concentration of CBC constant. The same final amplitude of fluorescentresponse was reached after 30 s of incubation, there-fore the reactions obeyed ... response for IFwas, however, three-fold higher (Table 1).Binding of CBC to IF fragments IF20or IF30The binding reactions were conducted at constantCBC and variable concentrations of the ... used concentrations of CBC £ 1 lm, where no self-quenching was observed,and the intensity of CBC fluorescence linearly depended on CBC concentration (data not shown). The excitationand emission...

Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

... experimental conditions occursin  1 month; therefore, in the 1 month time-periodbetween transfection and elimination, the parasite cul-tures are variable mixtures of transformed and untrans-formed ... linear conditions for protein concentra-tion and reaction time. ADC activities were calculated bymeasuring the radioactive CO2released during the reac-tions [24]. Protein concentrations of enzyme ... multimericform), only one of both PCR reactions should pro-duce a DNA segment of 2030 bp when using primersT7 (speciﬁc for the promoter region of pRIBOTEXvector) and ADC2 (speciﬁc for an internal...

Tài liệu Báo cáo khoa học: "Types of Common-Sense Knowledge Needed for Recognizing Textual Entailment" ppt

... organiza-tion, they belong to that organization.2. Preconditions: For a given action or event A attime t, a precondition p is a predicate that must holdtrue of the world before time t, in order for ... the κ of 0.640 for Mutual Exclusivity on its own. One merge thatimproves κ is a combination of Prominent Relation-ship with Argument Types (combined κ of 0 .250 , ascompared with 0.145 for Prominent ... textual entailment can do for you In Proc. of the Annual Meeting of the Association of Computa-tional Linguistics (ACL), Uppsala, Sweden, 7. Associ-ation for Computational Linguistics.Shachar...

Báo cáo khoa học: Improvement of ecdysone receptor gene switch for applications in plants: Locusta migratoria retinoid X receptor (LmRXR) mutagenesis and optimization of translation start site pptx

... Martinez A, Sparks C, Drayton P, Thompson J, Green-land A & Jepson I (1999a) Creation of ecdysone recep-tor chimeras in plants for controlled regulation of geneexpression. Mol Gen Genet 261, ... Department of Entomology, University of Kentucky, Lexington, KY, USA2 Plant and Soil Sciences Department, University of Kentucky, Lexington, KY, USAIntroductionThe conditional regulation of transgene ... robust induction of transgene expression in the presence of a low concen-tration (nanomolar) of a chemical ligand. In an effortto ﬁnd an ideal chemically inducible gene regulationsystem, several...

Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf

... agar.Construction of plasmids for yeast chromosomesubstitutionPlasmids used for deletion of STE18 gene by substitution of the kanMX4 gene (G418 resistance gene) on the yeastchromosome were constructed ... ratio in the detection of protein–protein inter-actions. Detection of interactions in the absence of ABCFig. 2. Schematic outline of gene construction. (A) Structural fea-tures of the yeast endogenous ... compilation ª 2009 FEBSConstruction of yeast strains containing Zmemgenesrather than the ZZmemgene were achieved using a processsimilar to construction of the BZG2118 strain, with theexception...

Báo cáo khoa học: Optimization of D-amino acid oxidase for low substrate concentrations – towards a cancer enzyme therapy pdf

... DAAO in the presence of high concen-trations of ammonia and pyruvate, conditions thatinduce formation of E-Flred$P (see Scheme 1b): in thiscase, the time course of reoxidation is clearly biphasic.A ... photoreduction method [18]. Inthe present case, near-complete formation of theanionic semiquinone (‡ 95% on the basis of ﬂavincontent) was found for wt-DAAO, Q144R-DAAO andm-DAAO. Semiquinone stabilization ... Comparison of the cytotoxicity of the dif-ferent DAAO forms on cultured N2C tumor cells, and dependence on the concentration of the substrateD-Ala. The effect wasobserved after 24 h of incubation...

Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf

... gene, using a combination of ligation andrecursive overlap extension of complementary syntheticoligonucleotides as described above. Oligonucleotides used for the construction of the s-CYPA are i ... transamination. In thissituation, the main effect of transamination is a reduction of the degre e of 15N-labelling in the amino a cid of interest. Inthecaseof[15N]Gly, much of the label ... vitro reaction mixture, but had littleeffect on protein yields.The concentration of tRNA was found to affect the yields of proteins. For production of aspartyl-tRNA synthetase[16], for example,...

Báo cáo khoa học: Activation of hepatocyte growth factor activator zymogen (pro-HGFA) by human kallikrein 1-related peptidases docx

... incubated for the same period without any cells is shown as ‘cell free’. hc, heavy chain of mature active form of HGF ⁄ SF. (B) Effects of engineered expression of KLK5 on cellular migration of SUIT-2. ... instructions.KLK3 and KLK4 were proforms, and required proteo-lytic activation using thermolysin followed by addition of phosphoramidon to inhibit excess thermolysin activitybefore use. One microgram ... Kataoka11 Section of Oncopathology and Regenerative Biology, Department of Pathology, Faculty of Medicine, University of Miyazaki, Japan2 Department of Urology, Faculty of Medicine, University of Miyazaki,...

Báo cáo khoa học: "Adaptation of Statistical Machine Translation Model for Cross-Lingual Information Retrieval in a Service Context" ppt

... Cross-language information retrieval us-ing meta-language index construction and structuralqueries. In Proceedings of the 10th cross-languageevaluation forum conference on Multilingual in-formation access ... of machine translation resources for english-japanese cross-language information retrieval. In InProceedings of MT Summit VII Workshop on Ma-chine Translation for Cross Language InformationRetrieval, ... Proceedings of the 2003 Conference of the North American Chapter of the Association for Computational Linguistics on Human LanguageTechnology, pages 48–54, Morristown, NJ, USA.Association for Computational...

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