Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc
... 2
Research article
The role of regulatory T cells in antigen-induced arthritis:
aggravation of arthritis after depletion and amelioration after
transfer of CD4
+
CD25
+
T cells
Oliver Frey
1
, Peter ... With this in mind, it
could be interesting to investigate whether the accumu-
lated T
reg
cells in patients with arthritis function properly...
... CAAAAGTCTTGGCGGACAAAATGAGGACTTGGTAC
Reverse CATTTTGTCCGCCAAGACTTTTGAATACTT TCAAGTGC
Q76G Forward CTTCCCGGAGGAAATGAGGACTTGGTACTTACTG
Reverse CCTCATTTCCTCCGGGAAGACTTTTGAATA C
N77G Forward CGGACAAGGTGAGGACTTGGTACTTACTGGATAC
Reverse ... contains three
domains resembling family 2 cystatins.
Cystatins competitively inhibit the activity of papain-
like cysteine proteases by binding to the active s...
... volume of the acyl binding pocket
and thereby alter the binding mode and affinity of the
enzyme for PAA and derivatives thereof, while maintaining
the hydrophobicity of the binding site.
To test the ... effect of the mutations on the specificity for
phenylacetylated substrates, the steady-state kinetic param-
eters for the hydrolysis of the chromogenic substrate
NIPAB and the...
... finding that both MtaA mutants still bind
coenzyme M and exhibit some activity although in both
mutants the coordination of zinc differs from the situation
in the wild-type enzyme. Apparently, other ... apparent
absence of any sulfur in the first coordination sphere of Zn
in the Cys239 fi Ala mutant suggests that Cys316 is not a
direct Zn ligand in MtaA. It would be interesting...
... [12,13]
suggest that correct orientation of the penicillin substrate
with respect to the putative high-energy ferryl intermediate
is important for optimum coupling between 2-oxoglutarate
and penicillin ... substrate, and may be a consequence of the higher K
m
value for this substrate [1,22]. The third type of mutant,
represented by arginines 306 and 307, are located in the
C-ter...
... as the timing of the
introduction of the probiotic, Taylor et al administered
the probiotic supplement postnatally, while other studies
administered probiotics before and after birth. Prenatal
supplementation ... in allergic disorders
The interest in probiotic therapeutic potential in allergic
disorders stemmed from the fact that they have been
shown to reduce inflammatory cytok...
... chal-
lenging. The recruitment of sufficient numbers of patients
to achieve statistical power, the development of reliable
methods to measure the bone repair endpoint, and the
randomization of patients ... the
doses of indomethacin and celecoxib used in the rats
were roughly equivalent to those used in patients, the
dose of rofecoxib was nearly eight times that used to
man...
... of
α-subunits, and two transactivating domains, namely the
amino-terminal transactivating domain and the carboxyl-
terminal transactivating domain (C-TAD). The C-TAD has
been shown to interact with co-activators ... proteins, and the intracellular
domain of the Notch receptor (involved in the maintenance of
cells in an undifferentiated state), both of which notably
contain...
... of TNF-α and IL-1 are other currently used
strategies. Both cytokines are potent osteoclastogenic
factors, produced in inflammatory arthritis. Interestingly,
clinical trials have shown that the ... 242
Osteoprotegerin as inhibitor of
osteoclastogenesis
Osteoprotegerin (OPG) has emerged as one of the most
attractive tools to inhibit osteoclast formation during the
past years. The in...
... apposition rate in the tibiae of rats
treated with cerivastatin at 0.1 mg/kg/day. Statins, there-
fore, have the potential to stimulate bone formation both in
vitro and in vivo in rats. Cerivastatin ... local
production of the osteogenic protein BMP-2. Interestingly,
pravastatin was unable to stimulate the BMP-2 promoter
activity and it did not stimulate new bone formation in...