Báo cáo khoa học: "The combination effect of sodium butyrate and 5-Aza-2''''''''-deoxycytidine on radiosensitivity in RKO colorectal cancer and MCF-7 breast cancer cell lines" pot

Báo cáo khoa học: "The combination effect of sodium butyrate and 5-Aza-2''''-deoxycytidine on radiosensitivity in RKO colorectal cancer and MCF-7 breast cancer cell lines" pot

... reagentsHuman colon cancer cell lines RKO (ATCC, USA), breast cancer cell line MCF-7 (KCLB, Korea), and normal colon cell line DDC-112 CoN (ATCC) were used. RKO and MCF-7 cell lines were cultivated in Dulbecco's ... the found in where effect of 5-aza-DC and SB were not measured underthe equal conditions.Conclusion5-aza-DC and SB enhanced radiosensitivity in MCF-7 and RKO cell lines. In RKO cell lines, which ... much information isknown about the combined effects of HDAC inhibitors and demethylating agents. In this experiment, humancolon and breast cancer cell lines were used to determinethe effects of...

Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt

... Comparison of the eﬀects of the LPSs of rough S. minnesota 595(Re) and smooth S. minnesota, in the absence and presence of EDTA, on the oxidation of a, a-cross-linked Hb. Themean±SDofthreeinde-pendent ... oxidation of the a chains. Therates of oxidation are reduced in the presence of chelators of heavy metal cations in most cases. An exception is the lack of alteration of the increased oxidation ... The participation of cupric ion in the oxidation process was demonstrated in most cases. In contrast, the rate of oxidation of cross-linked hemoglobin bythe LPSs of both the rough and smooth E....

Báo cáo khoa học: The antagonistic effect of hydroxyl radical on the development of a hypersensitive response in tobacco pot

... The antagonistic effect of hydroxyl radical on thedevelopment of a hypersensitive response in tobaccoSheng Deng1,2,*, Mina Yu1,2,*, Ying Wang1, Qin Jia1, Ling Lin2 and Hansong Dong11 ... Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agricuture of R. P. China, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural ... University, China2 Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaIntroduction In the course of their development, plants are oftenconfronted with various potential...

Báo cáo khoa học: " The inhibitory effect of quercitrin gallate on iNOS expression induced by lipopolysaccharide in Balb/c mice" pdf

... 0.05).approximately 77% of that of the expression of GAPDH in control cells. However, the expression of iNOS mRNA increased to 103% of that of the expression of GAPDH in control cells in response to treatment ... level of iNOS protein expression in control cells was approximately 25% of that of the expression of β-actin in the controls. In addition, iNOS protein expression in LPS treated mice increased ... expression of iNOS mRNA in the LPS group was 83% of that of the expression of GAPDH in the control group. Effect of QGR on iNOS protein expression in the liver As shown in Fig. 4, the level of...

Báo cáo khoa học: "The combined effect of erythropoietin and granulocyte macrophage colony stimulating factor on liver regeneration after major hepatectomy in rats" pot

... concentrations of hemopoietins havebeen found to play a key role in the final effect on cellularresponse [35].Conclusions In conclusion our data suggest that EPO and GM-CSF,when administered perioperatively ... regen-eration following major hepatectomy. Although the effect of EPO in this setting has already been reported, there arefew data on the effect of GM-CSF. In addition there areno data on the effect of ... showedincreased expression of PCNA in all groups (p < 0.01),while Ki-67 was increased only in the EPO treatmentgroup (p < 0.01). In all postoperative days, the combination of EPO and GM-CSF...

Báo cáo khoa học: "The radiosensitizing effect of Ku70/80 knockdown in MCF10A cells irradiated with X-rays and p(66)+Be(40) neutrons" potx

... blot of MCF10A cells after RNAi of Ku70. Protein expression levels of the Ku70 and the Ku80 protein are shown in both the LVTHM (control cell line) and Ku70i (RNAi of Ku70) cell line. Actin was ... of ionizingradiation depends on the quality of the radiation beam.Densely ionizing, high-linear energy transfer (LET) types of radiation are biologically more effective than sparselyionizing, ... the repair-deficient and -proficient cell lines [3,5,24] pointing to an involvement of the Ku protein in the repair of the radiation-induceddamage. When the radiation quality of the high-LETbeam...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... upregulation in the liver of streptozotocin-induced diabetic rats and the suppres-sion of such elevation following insulin injection[21,22], we decided to investigate the effect of NHCH2CHCOOHNH2L-kynurenineCHOCOOHCOOHNH2Tryptophanα-amino-β-carboxymuconate-ε-semialdehyde ... replacement and reﬁned at a resolution of 2.0 A˚. The monomerpresent in the asymmetric unit contains 332 residuesout of 336, one zinc ion, one DHAP molecule, one glyc-erol molecule and a total of ... is the main active sitegating determinant controlling ligand admission.ConclusionhACMSD appears to be an important enzyme control-ling the cellular levels of QA, PA and NAD. As aconsequence,...

Báo cáo khoa học: "The clinical implication of sodium-potassium ratios in dogs Son-Il Pak" pot

... medicalrecords included signalment, clinical signs on admission and historical findings, physical examination findings,results of biochemical analyses, information on concurrentdiseases, and outcome. In ... decreased production and lower fertility in large ruminants [20]. Normal plasma sodium and potassium concentrations are maintained bybalanced intake and excretion, intracellular and extra-cellular osmotic ... theextracellular compartment and a decrease in the renalexcretion of potassium. The former may be due to loss of the effects of cortisol upon the sodium- potassium pump,which normally maintains a potassium...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... the inhibitory effect of hr2 on transcriptional activation.Pbx interaction is separable from autoinhibitionThe Hth domain of Meis2 contains two regions, termedhr1 and hr2, which are highly conserved ... theBglII and HindIII sites, as previously described [18]. Dou-ble-stranded oligonucleotides containing either a consensusMeis2-binding and Pbx1-binding site or the Pbx–Hox-bind-ing site from ... ﬁrsttested the effects of targeting the GBD fusion proteinsto the TATA-containing luciferase reporter. As shown in Fig. 5B, none of these mutations resulted in signiﬁ-cant derepression of GDB–Meis2d....

Tài liệu Báo cáo khoa học: Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-ﬁn eelpout, Zoarces elongatus Kner ppt

... type IAFP isoforms from winter ﬂounder consisting of 11amino acid repeats, a longer isoform consisting of four repeats showed almost twice the activity of theordinary three-repeat isoforms [28]. ... residues located in theice-binding region. The less active SP isoform becomesactive on addition of a small amount of the activeQAE1 isoform, whereas it does not become active on addition of another ... annealing sites of theseprimers were connected to 3 ¢-terminus and 5¢-terminus of cDNA. The PCR conditions are as follows: denaturing at94 °C for 1 min, 2 cycles pre-extending at 94 °C for 1 min,extending...

Xem thêm