... sets for specificity (Fig. 1).Results showed that primer set of 3' NTR had good resultsbut the primer sets of prM and 5' NTR didn’t. Therefore,primers and probe of 3' NTR for the ... added toRT-mix for synthesis of cDNA. The RT-mix consisted of 6µl of 5X Universal buffer, 3µl of 0.1µl M DDT, 1µl of 10mM dNTP, 1µl of reverse primer (20 pM), 1 of Superscriptreverse ... mixture for each one tube TaqManreaction mix consisted of 5µl of Universal 5X buffer, 1µl of 10 mM dNTP, 1µl of enzyme mix (Qiagen, Germany), 1µl of 20 pM forward primer, 1µl of 20 pM...