... Biol142, 141– 151 .S. Sanchez et al. Carbonic anhydrase transcripts in RiftiaFEBS Journal 274 (2007) 53 11 53 24 ª 2007 The Authors Journal compilation ª 2007 FEBS 53 23 5. 55 cycles and 20.14 ± 0.34 ... 0 .5 lL of BSA (10 mgÆmL)1),1 lL of total RNA (1.24 lgÆlL)1), 2 .5 lL of dNTP (4 mmtotal), 1 .5 lL of Random Primer 9 (Ozyme, St-Quentin-en-Yvelines, France) (100 ngÆlL)1), 3 lL of diethylpyro-carbonate. ... overexpression approach of RpCAbr and RpCAtr, we cannot know the functionaleffect of changes of some key amino-acids.Origin and number of nonvertebrate CAsAlthough the bootstrap values of the deep branches...