... from inter-actions between proteins and the cofactor. From the viewpoint of coenzyme molecule itself, these interac-tions can be divided into three parts: the ribonucleo-tide tail, corrin ring ... Quantification of the axialbase effecting adenosylcobalamin by the synthesis and thermolysis of axial base-free adenosylcobinamide.Insights into the energetics of enzyme-assisted cobalt–carbon bond ... presence of 10, 25, 50, 75 and 120 lm cofactor, and the Km and kcatfor AdoCbi-GDPwere determined in the presence of 20, 70, 100, 150 and 200 lm cofactor. The reaction was initiated by addingl-glutamate...