... G. Oliveira et al .Stem growth of Quercus suber L.
Original article
The relative importance of cork harvesting
and climate for stem radial growth
of Quercus suber L.
Graça Oliveira
*
, ... slow growth of Q. suber, its large hetero
-
geneity (size, form, age) and the recurrent cork
harvesting. Here we present a first study of the effects of
c...
... with
AxCALSKD
EQ
at an MOI of 0, 1000, or 2000, and AxCAN-
Cre at an MOI of 150. After 24 h of incubation, the cells
were washed twice with NaCl ⁄ P
i
and fixed with 4% parafor-
maldehyde in NaCl ⁄ P
i
for 15 min. ... total amount of protein in cells decreased to 87%
after 48 h of incubation, the amount of glycoprotein
decreased more. The results of culturing for 48 h
sho...
... and GST-
lofMPK6. However, when lofMPK3 and lofMPK6
proteins were tested for phosphorylation with OXI1,
no phosphorylation of lofMPK3 or lofMPK6 was
observed (Fig. 5A). On the other hand, when ... 1% sucrose and 0.7% agar.
The seeds were stratified at 4 °C for 72 h and were then
transferred to 22 °C under long day conditions (16 h light,
8 h dark) for germination and growth....
... isolated isoforms of the bean PAL
after chromatofocusing are enzymes with increasing
number of Tyr-loop-out conformations at the four
active sites.
The isoelectric points of these forms can be ... of inactivation of PAL by enfor-
cing the Tyr-loop-out conformation which is catalyti-
cally inactive and more sensitive to degradation. The
presence of conformationally stable Tyr-loo...
... cycle of 95 °C for 2 min (Hot-
Start), followed by 10 cycles of 95 °C for 30 s, 58.7 °C for
30 s and 72 °C for 2 min, followed by 25 cycles of 95 °C
for 30 s, 58.7 °C for 30 s and 72 °C for 2 ... loss of CUX1-formed complexes, as observed for
sites 8, 10, 12, 13, 14 and 15 and, to a lesser extent,
for site 3 (Fig. 2B). The addition of an excess amount
of n...
... conception
and design of the study and substantially contributed to
interpretation of data, drafting of the manuscript, critical
revision of the manuscript and final approval. All authors
read and approved ... synthesized and provided ErPC and
ErPC3 for the analysis. WB significantly contributed to
data analysis. CB participated in the conception of the
study and int...
... to thank Kevin Howland and Judy Hardy
(Department of Biosciences, University of Kent, Can-
terbury, UK) for synthesis of the peptides, and the
Wellcome Trust for establishment of a Protein Science
Facility. ... per-
formed as described for SurA [11]. Cloning and protein
expression of human PDI were performed as described previ-
ously [11]. [
125
I]-Bolton–Hunter labellin...
... frequency-inverse document fre-
quency (Salton and Buckley, 1988), and log en-
tropy (Landauer and Dumais, 1997).
3.4 Measurements
The choice of similarity function for the vector
space is the least standardized ... of models approximate
31
Lin Dekang. 1998. Automatic retrieval and clustering
of similar words. In Proceedings of the Joint An-
nual Meeting of the Association...
... (see
Materials and methods) for 90 min, and
then incubated with and without NaF for an
additional 30 min at 30 °C before being
tested for interaction with 14-3-3. Lanes 1
and 2, 1% of the lysate used for ... implications for the respective roles of centaurin-a
and the 14-3-3 isoforms in the regulation of signalling
through CKI isoforms.
Materials and methods
Materi...
... for prmt1 morphants. (A) Dorsal view of embryos (10-somite stage) for krox20 staining.
The positions of r3 and r5 are indicated. The widths of r3 and r5 and the vertical distance between r3 and ... to form the precursors of the meso-
derm and endoderm, and C ⁄ E, in which cells accumu-
late on the dorsal side and lead to axis formation.
Gastrulation begins at 50% of...