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Báo cáo khoa học: "Two different types of malignant fibrous histiocytomas from pet dogs" docx

Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt

Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt

... discovery of a specific post-translational cleavage of recombinantly expressed nitrilase from R. rhodochrous J1 which leads to the for-mation of stable, active helices. 3D reconstruction of the negatively ... Yamamoto M,Kumasaka T, Ueki T, Nanba H, Ikenaka Y, TakahashiS, Sato M et al. (2000) Crystal structure of N-carbamyl-d-amino acid amidohydrolase with a novel catalyticframework common to amidohydrolases. ... the active fraction showed a characteristic nitrilase band of  40 kDa. The contaminating band at60 kDa was identified as GroEL on the basis of its characteristic appearance in the micrographs....
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Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx

Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx

... background of at least1000 subjects was typical of the American Midwest with 90% of European ancestry and  5% each of Africanand Asian ancestry. An exception was the targeted analysis of ... its altered N-terminal truncationon the metabolic functions of ApoC1.AbbreviationsApoC1, apolipoprotein C1; ApoC2, apolipoprotein C2; ApoC3-0, ApoC3 that does not contain a carbohydrate chain; ... chain; ApoC3-1, ApoC3 with a GalNAc-Gal-sialic acid carbohydrate chain; ApoC3-2, ApoC3 containing the carbohydrate of ApoC3-1 plus an additional sialic acid residue;DPPase, dipeptidylpeptidase...
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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... Journal 272 (2005) 45494557 ê 2005 FEBS Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded ... Medium chain acyl-CoA dehydrogenase (MCAD) is a homotetrameric flavoprotein that catalyses one of therecurrent steps in the b-oxidation of fatty acids.MCAD functions by removing two reducing ... well-folded but totally inactive protein Linda P. O’Reilly1,*, Brage S. Andresen2 and Paul C. Engel11 Department of Biochemistry and Conway Institute of Biomolecular and Biomedical Research, University...
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Báo cáo khoa học: Protein folding intermediates of invasin protein IbeA from Escherichia coli pdf

Báo cáo khoa học: Protein folding intermediates of invasin protein IbeA from Escherichia coli pdf

... Gdm-HCl-induced unfolding; Escherichia coli; molten globule; protein unfolding intermediates of IbeA CorrespondenceK. S. Kim, Division of Pediatric InfectiousDiseases, Johns Hopkins University School of Medicine, ... isavailable on protein folding intermediates [11–18],there are no reports available for E. coli invasin pro-tein IbeA. The process of unfolding and refolding isuseful for a complex unfolding transition, ... have shown the existence of protein folding intermediates and acidic unfolding intermedi-ates in vitro.Purification of IbeA The expression level of IbeA was 68 mgặL)1 of culture, and its molecular...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... domains. The largest domain encompasses strands b1 to b6 a nd strand b7 to the C-terminus. The central b sheet iscomposed of 7 parallel b strands associated to an anti-parallel strand (b2) and is ... of Rv1399c from M. tuberculosis (Eur. J. Biochem. 271) 3955 Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related ... His290 and Asp260 e merge from the b8 -a8 andb7 -a7 loops, respectively. Anotherimportant feature of the catalytic machinery is the so-calledoxyanion hole necessary for the stabilization of the oxyan-ion...
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Báo cáo khoa học: Molecular identification of monomeric aspartate racemase from Bifidobacterium bifidum pptx

Báo cáo khoa học: Molecular identification of monomeric aspartate racemase from Bifidobacterium bifidum pptx

... proteinsequencer.ResultsPurification of aspartate racemase from B. bifidum The aspartate racemase was purified to homogeneity from cell extracts of B. bifidum NBRC 14252. A summary of thepurification is presented ... Linear alignment of amino acid se-quences of PLP-independent aspartate race-mases. Bb, the monomeric aspartate racemase from B. bifidum; St, the dimeric aspartate racemase from S. thermophilus ... we succeeded inidentifying the aspartate racemase from B. bifidum .Enzy-mological properties of aspartate racemase purified from B. bifidum NBRC 14252, such as cofactor independencyand susceptibility...
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Báo cáo khoa học:

Báo cáo khoa học: "Improving the Use of Pseudo-Words for Evaluating Selectional Preferences" docx

... no-table since the Google model only requires n-gramcounts to implement. The Backoff Erk model is the best, using the Baseline for the majority of decisions and backing off to the Erk smoothingmodel ... is the concatenation of twowords (e.g. house/car). One word is the orig-inal in a document, and the second is the con-founder. Consider the following example of ap-plying pseudo-words to the ... which of two verbswas the correct predicate for a given noun object.One verb v was the original from the source doc-ument, and the other vwas randomly generated.This was the first use of such...
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Báo cáo khoa học: Calpain-mediated degradation of reversibly oxidized protein-tyrosine phosphatase 1B docx

Báo cáo khoa học: Calpain-mediated degradation of reversibly oxidized protein-tyrosine phosphatase 1B docx

... hydrogen peroxide reversibly inhibits protein-tyrosine phosphatase 1b in vivo and enhances Calpain-mediated degradation of reversibly oxidized protein-tyrosine phosphatase 1B A. Truămpler et ... fragment, consistent with the lack of a part of the catalytic core (see Fig. 3). Calpain-mediated degradation of reversibly oxidized protein-tyrosine phosphatase 1B A. Truămpler et al.5624 FEBS ... PTP1B. Representativeexamples of at least three independent experiments are shown.A. Truămpler et al. Calpain-mediated degradation of reversibly oxidized protein-tyrosine phosphatase 1B FEBS...
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Báo cáo khoa học: Does different orientation of the methoxy groups of ubiquinone-10 in the reaction centre of Rhodobacter sphaeroides cause different binding at QA and QB? potx

Báo cáo khoa học: Does different orientation of the methoxy groups of ubiquinone-10 in the reaction centre of Rhodobacter sphaeroides cause different binding at QA and QB? potx

... downshift of the 4CẳO stretching vibration of QAby 60 cm)1[10,11], indicating strong asymmetric binding of UQ10 at the QAsite, in contrast to symmetric,weaker binding of UQ10 at the QBsite ... reaction centre of Rb. sphaeroides should provide the methoxy orientations of UQ10 at the QA and QB binding sites.However, there are a large variety of contradicting confor-mations in the different ... QA and the QB binding sites.Therefore, we conclude that the same conformation ispresent in unbound UQ10andinprotein-boundUQ10 at the QA and QB binding sites. The agreement with the abovecalculations...
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Báo cáo khoa học: Functionally different pools of Shiga toxin receptor, globotriaosyl ceramide, in HeLa cells doc

Báo cáo khoa học: Functionally different pools of Shiga toxin receptor, globotriaosyl ceramide, in HeLa cells doc

... thechange of the molecular species composition of the cel-lular Gb3[28,29]. In in vitro binding assays, the fattyacid chain of Gb3was found to in uence the bindingto Shiga toxin [30,31]. In this ... clathrin functionhas minimal effects on Shiga toxin endocytosis, show-ing that Shiga toxin can enter cells efficiently via clath-rin-independent endocytic mechanisms. As opposed toits endocytosis, ... for Shiga toxin binding to cells, very few studies have aimed atinvestigating the lipid directly. A correlation has beendescribed between the sensitization of cells to Shiga toxin following...
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Báo cáo khoa học: Two different types of hepcidins from the Japanese flounder Paralichthys olivaceus ppt

Báo cáo khoa học: Two different types of hepcidins from the Japanese flounder Paralichthys olivaceus ppt

... is different from that of human,mouse and other reported fish hepcidins. In liver, somecells expressed both types of hepcidins, while othercells expressed only one of them or neither of them(Fig. ... plated on the appropriate agar platesand incubated for 24 h.Hemolytic activity of synthesized Japanese floun-der hepcidins The hemolytic activity of the synthetic Japanese flounder hepcidins ... hepcidin of winter flounder. Both genesexist as single copies in the Japanese flounder genome. The structures, and therefore the functions, of Hep-JF1and type II hepcidin of winter flounder might...
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Báo cáo khoa học:

Báo cáo khoa học: "Two different types of malignant fibrous histiocytomas from pet dogs" docx

... +82-53-950-5975; Fax: +82-53-950-5955E-mail: jeongks@knu.ac.krTwo different types of malignant fibrous histiocytomas from pet dogsSun Hee Do1, Il-Hwa Hong2, Jin-Kyu Park2, Ae-Ri Ji2, ... are indeed from a primitive cell type or are the end result of “differentiation” of several different types of sarcomas [4,8]. The undifferentiated mesenchymal cell-origin tumors in which giant ... Two different types of malignant fibrous histiocytomas 171histiocytoid cells. Inflammatory-type tumors showed bizzare...
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báo cáo khoa học:

báo cáo khoa học: " Two-stimuli manipulation of a biological motor" ppsx

... rotation of F(1)-ATPaseduring catalysis. Proc Natl Acad Sci 2001, 98(24):13649-13654.20. Paik SR, Yokogama M, Yoshida M, Ohta T, Kagawa Y, Allison WS:The TF1-ATPase and ATPase Activities of Assembled ... Indiana, USA). Other chemicals were of thehighest grade commercially available.Preparation of F1-ATPaseThe α3β3γ core complex of F1-ATPase originating fromBacillus PS3 was prepared as ... of distinct rotational substeps by submillisecond kinetic analy-sis of F1-ATPase. Nature 2001, 410:898-904.8. Itoh H, Takahashi A, Adachi A, Noji H, Yasuda R, Yoshida M, KinositaK: Mechanically driven...
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báo cáo khoa học:

báo cáo khoa học: " Two novel types of hexokinases in the moss Physcomitrella patens" ppsx

... hexokinases have lost intron 2,which is present in the other moss hexokinases, includ-ing the type D hexokinases (Figure 1). Finally, we notethat the sequence of the type C hexokinase, PpHxk4, ismore ... of the Physcomitrella hexokinases We proceeded to study the intracel lular locations of the moss hexokinases. Sequences from the new hexokinases, expressed from the 35S promoter, were fused in ... also involved in sugarsensing in both fungi and plants. We have previously described two types of hexokinases in the moss Physcomitrella. Type A, exemplified by PpHxk1, the major hexokinase in Physcomitrella, ...
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