Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... here.Purification of four pulchellin isoforms from A. pulchellus seeds Using a combination of affinity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A. pulchellus ... four pulchellin isoforms P. V. Castilho et al.954 FEBS Journal 27 5 (20 08) 948959 ê 20 08 The Authors Journal compilation ê 20 08 FEBS Isolation and characterization of four type 2 ribosome inactivating ... Araujo AP (20 05) Pulchellin, ahighly toxic type 2 ribosome- inactivating protein from Abrus pulchellus. Cloning heterologous expression of A-chain and structural studies. FEBS J 27 2, 120 1– 121 0.16...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... proceduresIsolation of the tyrosinase gene from Trichoderma reesei The tyr2 gene was amplified from genomic T. reesei DNAwith the following primers: forward, GGG GAC AAGTTT GTA CAA AAA AGC AGG CTA TCA TGC ... TGTCAG GTC CCT CTC G; and reverse, GGG GAC CACTTT GTA CAA GAA AGC TGG GTC A GT GGT GGTGGT GGT GGT GCA GAG GAG GGA TAT GGG GAACGG CAA A. The PCR reaction was done with the Dyna-zyme EXT thermostable ... Casalot L, Hamdi M,Sigoillot J-C, Asther1 M & Lomascolo A (2006) Cloning and characterization of a tyrosinase gene from the white-rot fungus Pycnoporus sanguineus, and overproduction of the...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... forward)45Â-GTATCAGCTTC(C T)TCAAATGTC-3Â PsCBL (degenerate reverse)55Â-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5ÂUTR forward)65Â-TTAAGTACTATAAAT-ACACAGCCTA-3Â PsCIPK (3ÂUTR reverse)75Â-CGAGCTCACTGCCTCTCAAC-3Â ... T)AAGGT-3Â PsCIPK (degenerate forward)25Â-ACAAA (A C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C G)ACACCACAAGACC)3Â PsCIPK (degenerate reverse)35Â-CTTAT(C G)AACAAGGAA (A C)AATTTC-3Â PsCBL (degenerate ... nucleolus and sti-mulated by phosphorylation with CK2 and cdc2 proteinkinases. Plant J 25, 9–17.32 Yadav N, Chandok MR, Prasad J, Bhattacharya S,Sopory SK & Bhattacharya A (1997) Characterization Stress-induced...
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... for existence of two distinct HSF1 isoforms in rainbow trout and the formation of heterotrimers of these isoforms in vitro.Materials and methodsCell culture and animals Rainbow trout gonadal ... encode distinct isoforms of HSF. Clone C1 wasa partial cDNA containing an insert of 983 nucleotidesÓ FEBS 2004 Rainbow trout HSF1 (Eur. J. Biochem. 2 71) 705 Cloning and characterization of two distinct ... transcription factors in regulation of the heat shock response and beyond. FASEB J. 15 , 11 18 11 31. 5. Mosser,D.D.,Heikkila,J.J.&Bols,N.C. (19 86)Temperatureranges over which rainbow trout fibroblasts...
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Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
... FEBS 5907 Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters Ilka Knuătter1, Bianka Hartrodt2,Geza Toth3, Attila Keresztes3, ... Gly-Sar,Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala,d-aminolevulinic acid, cefadroxil and Ala-4-nitroanilide(all 100 lm, Table 1). Glycine, which is not a substrate of peptide transporters, ... the design, rational synthesis and functional characterization of a novel radiolabeled hydrolysis-resistant high-affinity substrate for H+⁄ peptide cotransporters. l-4,4Â-Biphenylalanyll-Proline(Bip-Pro)...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition Karuppiah Chockalingam1,*, James Luba2,*, Harry S. Nick3, David N. Silverman2 and Huimin ... rela-tionships of human manganese superoxide dismutase. AbbreviationsFeSOD, iron superoxide dismutase; hMnSOD, human manganese superoxide dismutase; MnSOD, manganese superoxide dismutase. FEBS ... anti-tumor effects of anactive site mutant of human manganese- superoxide dismutase. Evolutionary conservation of product inhibi-tion. J Biol Chem 279, 12769–12776.19 Bull C, Niederhoffer EC, Yoshida...
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot
... the mistletoe toxic lectin genes. Like other type 2 RIPs (such as ricin and abrin) [2], the mlp genes do not contain introns and they encode the toxic lectins in the form of a single chain precursor ... (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricinB-chain Leu152; and Met234 of the ML1p and ML2pB-chains corresponding to the ricin B-chain Leu233).Estimation ... Asn255 and Gln256 of the ricin B-chain in the second sugar binding site [28]. The exceptions are the replacement of Trp38 fi Ser in the ML2p B-chain and Tyr252 fi Phe in the ML3p B-chain (Fig. 2).The...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... domains. The largest domain encompasses strands b1 to b6 a nd strand b7 to the C-terminus. The central b sheet iscomposed of 7 parallel b strands associated to an anti-parallel strand (b2) and is ... of Rv1399c from M. tuberculosis (Eur. J. Biochem. 271) 3955 Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related ... His290 and Asp260 e merge from the b8 -a8 andb7 -a7 loops, respectively. Anotherimportant feature of the catalytic machinery is the so-calledoxyanion hole necessary for the stabilization of the oxyan-ion...
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Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii Implications for the disulfide metabolism of anaerobic hyperthermophiles doc
... (5Â-GGCCTCATGAAGAAAAAGGTCGTCATAATT-3Â), and TG101 (5Â-GGCCAAGCTTCTAGAACTTGAGAACCCTAGC-3Â) (for the P. horikoshii CoADR), and TG104 (5Â-CGCGCCATGGAAAAGAAAAAGGTAGTCATAA-3Â) and TG105 (5Â-CGCGGTCGACCTAGAACTTCAAAACCCTGGC-3Â) ... constantobserved for the CoADR reaction.Thermostability and thermoactivity of the CoADRphCoADR is stable for months at both )80 °C and )20 °C, and has half-lives of > 100 and 39 h at 85° and 95 °C, ... lL. Oxidation of NAD(P)H was monitored at 340 nm. The standard oxid-ase assay used to monitor the purification of phCoADRwas conducted with 100 lm NADH and FAD as above. A unit of CoADR activity...
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Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of specific proteins and functions docx
... FEBS proteins were detected in the HD -caveolae and LD -caveolae, particularly in the LD -caveolae, but therewas no labelling coinciding with the VHD -caveolae (Fig. 2D). As the sulfo-NHS-biotin reagent ... (Fig. 2G), but increased in responseto insulin in both the LD -caveolae and HD -caveolae, with the increase being particularly pronounced in the LD -caveolae (Fig. 4C). The presence of the triacylglycerol-hydrolysingenzyme ... to insulin, the insulin receptor autophosphorylation and the amount of insulin-regulated glucose transporter-4 increased in these caveolae. The molar ratio of cholesterol to phospholipid in the...
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Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx
... iron- and zinc-containing alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furio-sus. J Bacteriol 181, 1163–1170.12 Shimizu Y, Sakuraba H, Kawakami R, Goda S, Kaw-arabayasi ... similaritywith (putative) TDHs and zinc-containing alcohol de-hydrogenases from archaea and bacteria. Some of the most significant hits of a BLAST search analysis were a TDH from Pyrococcus horikoshii ... thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus Ronnie Machielsen and John van der OostLaboratory of Microbiology, Wageningen University, the Netherlandsl-Threonine...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana Anja Riemenschneider, Rosalina Wegele, Ahlert Schmidt and Jutta PapenbrockInstitute for Botany, ... Nagasawa T, Ohkishi H, Kawakami B, Yamano H,Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-ala-nine chloride-lyase (deaminating) of Pseudomonas putidaCR 1–1. Purification and characterization ... sulfur-transferases from Arabidopsis thaliana. Plant Physiol135, 916–926.26 Burandt P, Papenbrock J, Schmidt A, Bloem E, Hanekl-aus S & Schnug E (2001) Characterization of Brassicanapus L....
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Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt
... FEBS Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding Lucı´ a Garcia´-Ortega1, Javier Lacadena1, ... Ct -Aspf1 (5Â-GTCGTCTTGGATCCTCTCGAGTCTCAATGAGAACACAGTCTCAAGTC-3Â). These primers contained BstEII and BamHI sites and were used to generate a fragment that wascloned in the same sequencing and ... ananti -Aspf1 antiserum, of Aspf1 (lane 1), and Aspf1D(7–22) (lane 2). The samples were previously reduced with 5% (v ⁄ v) 2-mercapto-ethanol and boiled for 20 min.Variants of the A. fumigatus...
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Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf
... Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages Yuichiro Otsuka1, Tomonori ... of 18Ofromradio-labeled water was not observed with guaiacol.It was clear that the b-aryl ether cleavage enzyme catalyzed the addition of two molecules of H2O(atCa and Cb positions) and cleavage the b-aryl ... enzyme cleaved the b-aryl ether linkages of DHP-GOU, a high-molecular mass compound (Fig. 3B).Purification and characterization of the b-aryl ether cleavage enzyme To characterize in further detail the...
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Từ khóa: Nghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Thơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ