... nitrogen nuclei both of
15
NO
and of
14
N-proximal ligand cause the splitting. The
heme proximal ligand is considered to be His16, which
corresponds to His17 of the proximal ligand of heme-
Syn HO-1. ... characterization of the cyanobacterial
HO-2 protein and its heme complex.
Results and Discussion
Expression and purification of Syn HO-2
Culturing the cells at two temperat...
... Kruus
2
,
Juha Rouvinen
1
and Nina Hakulinen
1
1 Department of Chemistry, University of Eastern Finland, Joensuu, Finland
2 VTT Technical Research Centre of Finland, Espoo, Finland
Keywords
ascomycete; ... b-bar-
rels of domains A and B (Fig. 2B), and had six hydro-
gen bonds, three to the main chain carbonyls at Asn6,
Leu168, and Val170, and three to the side chains of
Ar...
... Hospital and University of New South Wales, Sydney, NSW, Australia
4 School of Chemistry and Molecular Biosciences and Centre for Infectious Disease Research, University of Queensland, Brisbane, ... importin-a recognition of the
NLS and is conserved in all human CLICs, except
CLIC3. The significance of the motif is clear, with
92% of the hydrogen bonds formed between impo...
... (Fig. 3A). Three of the ligands,
OD2 of Asp165, NE2 of His79 and NE2 of His169,
formed an equatorial plane. The other protein ligand,
NE2 of His31, bound to the metal, in the company of
a water oxygen, ... coordi-
nated with six ligands: the five same ligands in the
Mn-reconstituted enzyme and an additional water oxy-
gen, which, together with the OD2 of Asp165, the...
... crystal structures of
the ligand-free PhyK at 1.7 A
˚
resolution and of a cata-
lytically incompetent PhyK mutant that suggest a
model for substrate binding. Comparison with the
structures of the ... compilation ª 2010 FEBS
mutagenesis of phyK. The H25A mutation was confirmed
by sequencing analysis.
Gene expression and purification of PhyK
The genes encoding PhyK and the m...
... determined the structure of HtA by NMR methods. The
structure consists of one a-helix, a helical turn and seven b-strands that
form an N-terminal hairpin and an anti-parallel b-sheet, with a characteris-
tic ... FEBS
involvement of a catalytic pair constituted by an acid
and a base on each side of the hydrolyzed bond [16].
E96 and H137 in a-sarcin and E58 and H...
... shows
excellent stereochemistry, with 98.5% of all residues in the
most favored and favored regions of the Ramachandran
plot and r.m.s.d. values for bond and angle of 0.005 A
˚
and
1.37 ° as shown in Table ... crystal structure of the complex
of this protease with benzamidine.
Results and Discussion
Overall structure
The catalytic domain of DESC1 resembles an o...
... hydrogen-bon-
ded antiparallel b-strands (b6–b7), an a-helical hairpin
(helices a5, a6) and a C-terminal strand (b8). Helix a5
and strand b7 partially cover one side of the Ross-
mann fold structure, ... three-stranded b-sheets, each one consisting of
the antiparallel b-strands b6, b7 of one monomer and
strand-b8 of the other. Solvent-accessible surface [15]
calculations sho...
... B area,
protection of the nucleosome C area and repositioning of
initially randomly positioned nucleosomes (Fig. 5A and B,
compare lanes 1, 2, and 3, 4 and corresponding scans). Quite
unexpectedly, ... TSA
administration has on chromatin structure and on gene
expression.
Materials and methods
DNA and plasmids
Construction of the MMTV reporter and the plasmid for
in v...