Báo cáo khoa học: "Characterization of Brachyspira hyodysenteriae isolates from Korea" potx

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... 1343 Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas Anna Maria Luciani1, Sveva Grande1, Alessandra Palma1, Antonella Rosi1, Claudio Giovannini2,Orazio ... 1–12.8 Santini MT, Romano R, Rainaldi G, Ferrante A,Motta A & Indovina PL (2006) Increases in 1H -NMR A. M. Luciani et al.1H NMR of mobile lipids in tumour cells FEBS Journal 276 (2009) ... characteristic cross peak from the geminalprotons of carbons 1 and 3 of glycerol in TG [1].Cross peaks of lipids, including the T signal, wereabsent in cells characterized by low ML signals in 1Dspectra...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... of the soleGlyco_18 domain, the C-terminal tail of C. gigas CLPsmay not noticeably contribute to the structure and the function of these proteins. Interestingly, Cg-Clp1 and Cg-Clp2 C-terminal ... Oyster chitinase-like proteins FEBS Journal 274 (2007) 36463654 ê 2007 The Authors Journal compilation ª 2007 FEBS 3651 Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in ... 2007)doi:10.1111/j.1742-4658.2007.05898.x Chitinase-like proteins have been identiﬁed in insects and mammals as non-enzymatic members of the glycoside hydrolase family 18. Recently, the ﬁrstmolluscan chitinase-like protein, named Crassostrea...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... 3097 Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation Yoshihiko ... dele-tion of 39 bp. The thick lines indicate intron 6 of 75 bp in fragmentsB and C, 3Â portion of 35 bp in intron 5 in fragments C and D, and 3Â portion of 25 bp in intron 6 in fragments F and I. ... Department of Legal Medicine and Medical Genetics, Gunma University, Japan2 Division of Medical Genetics and Biochemistry, University of Fukui, Japan3 Division of Legal Medicine, University of Fukui,...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... plasma membrane receptor and the activation of the plasma membrane H+-ATPase. IV. Fusicoccin induces the association between the plasma membrane H+-ATPase and the fusicoccin receptor. Plant ... et al.5866 FEBS Journal 272 (2005) 58645871 ê 2005 FEBS Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado ... Soave C & De MichelisMI (2002) A novel interaction partner for the C-termi-nus of Arabidopsis thaliana plasma membrane H+-ATP-ase (AHA1 isoform): site and mechanism of action onH+-ATPase...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... 5Â-CTTTAACTTGTTGGGCACTGGCATTG-3Â; NP6, 5Â-TTGATCGATTCTGTCTATGCCCCA-3Â along with the adaptor primers: AP1 (5Â-GTAATACGACTCACTATAGGGC-3Â) and AP2 (5Â-ACTATAGGGCACGCGTGGT-3Â).Nested PCR was carried ... obtain the full length sequence:OP5, 5Â-GACTGTAACGGTCATGGYACMAYGT-3Â;OP6, 5Â-GATGAAAATCCTAACCTCTCCCCCGCACAG-3Â; OP7, 5Â-ACTGCACCTACGGCGGGTCGTTGGTACGTG-3Â; NP4, 5Â-GACACCGTAGGTTGAGCCGCCAATCGTCCC-3Â; ... PCR was performed in 50 lL containing 1 ng of genomic DNA as template, 0.2 mm dATP, dCTP, dGTPand dTTP, 0.2 lm of upstream primer (OP17: 5Â-GAAAAACCATGGTGAATGAATACCAAGCGACT-3Â ) anddownstream...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... 9th cycle from the N-terminusincluding the initiating Met: MHKTHSTMS for Sno1p and MTGEDFKIKS for Snz1p.Properties of the complex of Sno1p and Snz1pWhen Sno1p with a His-tag and Snz1p without ... Presumably, their homologues SNO2,SNO3, SNZ2 and SNZ3 complement the defect. The relationship of all of these genes remains to be clariﬁed.Expression and puriﬁcation of Sno1p and Snz1p In light of the ... of the genes. In SNO1, the 199th G from the 5Â terminus of the open readingframe was deleted to result in a frame-shift and appearance of stop codons in the downstream. As a result, a protein...

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... pGL3e:Prm3AP)1*, pGL3b: Prm 3a AP)1*, pGL3e:Prm 3a AP)1*,pGL3b:Prm3abAP)1*, pGL3e: Prm3abAP)1*, pGL3b:Prm3aaAP)1*, pGL3e:Prm3aaAP)1*, pGL3b:Prm3aaaAP)1* and pGL3e:Prm3aaaAP)1*.Mutation ... 1047 Characterization of promoter 3 of the human thromboxane A 2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity Adrian T. Coyle and B. Therese KinsellaDepartment ... (5Â-CACCAGAGCTACTTACACTGAATTCCAGAATAATCACAAGCAAATC -3 ; senseprimer) vs. its complement generating pGL3b:Prm 3a OCT)1*,pGL3e:Prm 3a OCT)1*, pGL3b:Prm3abOCT)1* and pGL3e:Prm3abOCT)1*.Mutation of the...

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase Sabina Passamonti1, Alessandra Cocolo1, ... uptake inhibition by antibodies The kinetics of bilitranslocase transport activity inhibition by antibodies were examined by preincubating 24 lL rat liverplasma membrane vesicles or carnation petal ... carrier and theligands [39]. The occurrence of this effect was alsoinvestigated in carnation petal microsomes by preincu-bating them with antibody A in the presence of increasing concentrations of...

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... specifically with the I domains of CD11a/CD18 and CD11b/CD18 integrins. The binding was inhibited by anti -I domain and anti -ICAM-4 antibodies and it was dependent on divalent cations. Inter-estingly, ICAM-4 ... dose-dependently to isolated recombinantCD11a and CD11b I domains. The effective inhibition of binding of ICAM-4 positive red cells by anti -ICAM-4 antibodies, indicate a major role for ICAM-4 in binding of red ... cells to the I domains. The efficient inhibition of the Fig. 10. Specific binding of purified recombinant I domain GST fusionproteins to ICAM-4Fc in a solid phase assay. Dose-dependent binding of...

Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt

... the encoded protein. The gene was cloned from the methanotrophic Gram-negative bacterium Methylococcus capsulatus (Bath) and encodes a pro-tein of 131 amino acid residues. The prokaryotic haemerythrin ... mM NaCl, 0.4 mMAEBSF-hydrochloride). Characterization of prokaryotic haemerythrin O. A. Karlsen et al.2436 FEBS Journal 272 (2005) 24282440 ê 2005 FEBS Characterization of a prokaryotic haemerythrin ... N-terminally or MS-identiﬁed spots.Approximate molecular masses and pI values are indicated to the left and at the top of the gels, respectively. Characterization of prokaryotic haemerythrin O. A. ...

Báo cáo khoa học: Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli pptx

... nonheatedsamples, the mature form of GluV8 could be renaturedeven after exposure to heat in the presence of SDS.Role of N-terminal Val69 in processing of the GluV8 proformFinally, the role of N-terminal ... postulated in the experiment of Fig. 2.Mutation of the essential amino acid Ser237Establishment of the E. coli expression system of GluV8 enabled the roles of certain amino acids com-prising the ... active. This mayinduce the cascade reaction of GluV8 activation,because recombinant proteins remain inside E. coli cells, instead of being secreted from S. aureus. The conversion of amino acids adjacent...

Báo cáo khoa học: Characterization of c-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana pptx

... 2003 Characterization of c-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana Maria Koch1,2, Rainer Lemke3, Klaus-Peter Heise2 and Hans-Peter Mock11Institute of ... detailed biochemical characterization of a c-tocopherol methyltransferase (c-TMT) from Arabidopsis thaliana and of a c-TMT purified from Capsicum annuum fruits, a tissue with high accumulation of ... 2-methyl-6-phytylquinol, the first ring methy-lation at position 3 to yield 2,3-dimethyl-5-phytylquinol,cyclization to yield c-tocopherol, and finally a second ringmethylation at position 5 to yield...

Báo cáo khoa học: Characterization of a digestive carboxypeptidase from the insect pest corn earworm (Helicoverpa armigera) with novel speciﬁcity towards C-terminal glutamate residues pptx

... identity),rather than identical with the N-terminal region of H. armigera carboxypeptidase A. Identiﬁcation of cDNAs encoding carboxypeptidasesinH. armigeralarval gut library The characterization of ... was assayed, there was a qualitative correlationbetween the appearance of the putative 36 kDa activated carboxypeptidase polypeptide, and the level of activityÓ FEBS 2004 Glutamate- speciﬁc insect ... trypsin, the carboxypepti-dase activity is maximal. Characterization of recombinant HaCA42 carboxypeptidase activity The pH optimum for hydrolysis of FAEE by the activatedHaCA42 carboxypeptidase was...

Báo cáo khoa học: "Characterization of Brachyspira hyodysenteriae isolates from Korea" potx

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báo cáo khoa học: " Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen" pot

... n.q.Xa+g+c: 82XVIIa: 10 0- 4–5%01B 01 1B Ib: 69 IIIb: 19 IVa: 10 0 Vb: 23 VIIa: 75 VIIIc: n.q.Xb: 18 XVIIa: 10 0 18 19 % -01C04/01C051C Id: 31 IIIa: 51 IVa: 10 0 Va: 46 VIIa: 75 VIIId: 10 0Xa+g+c: ... distinguished Bet v 1 isoforms with alow, intermediate and high IgE-reactivity. Expression of the isoforms 01B 01 (= Bet v 1d, low IgE-reactivity), 02C 01 (= Bet v 1c, intermediate IgE-reactivity), ... Bet v 1 fragments. The higher band at 19 kDacontained birch cyclophilin (Bet v 7; CAC8 411 16; 3 pep-tides, coverage 28%) and some minor traces of Bet v 1 (Bet v 1a; CAA33887; 1 peptide, coverage...

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