Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... (2005),/6(1), 7–19Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPDJi-Yeon Kim1,2, So-Hyun ... wereapplied for further determination by multiplex PCR and PCR for flagellar H7 antigen detection. DNA preparation for Multiplex PCR, flagellar H7 PCR and RAPD analysisE. coli O157:H7 strains which ... of them did not agglutinateagainst H7 antiserum.Characterization of E. coli O157:H7 isolates by multiplex PCR for Stx1, Stx2, eaeA, and hlyA genes, and by flagellar H7 PCR After identification...
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Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf
... wereanalyzed by autoradiography and silver staining. Equalmobilities of the radioactive and the protein-containingbands indicated covalent attachment of DNA to theenzyme. The yield of the photochemical ... action of baserelease by Escherichia coli Fpg protein: role of lysine 155 in cata-lysis. Biochemistry 36, 5084–5096.21. Smith, R.A.G. & Knowles, J.R. (1975) The preparation and photolysis of ... as the ratio of the covalentconjugate radioactivity to the total radioactivity of theconjugate and unbound DNA.Fig. 1. Structures of (A) oligonucleotides and modified DNA duplexes and (B) modified...
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Báo cáo khoa học: Chaperone-assisted refolding of Escherichia coli maltodextrin glucosidase doc
... Government of India, and MHRD, Government of India, for financial support.We also thank Professor Winfried Boos, University of Konstanz, Germany, for the gift of plasmids encodingHis-tagged MalZ and ... refolding of Escherichia coli maltodextrin glucosidaseSubhankar Paul1,2, Shashikala Punam1 and Tapan K. Chaudhuri11 Department of Biochemical Engineering and Biotechnology, Indian Institute of ... denature MalZ and the denaturation was monitored by measuring the loss of biological activity of MalZ. Optimized concentration of different chemical chaperones in the denaturationmixture and, in every...
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Báo cáo khoa học: Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy pot
... of rates and yields of interchromophore (folate fi flavin) energy-transfer and intermolecular (flavin fi DNA) electron-transfer in Escherichia coli photolyase by time-resolved fluorescence and absorption-spectroscopy. ... bands at 1532 and 1396 cm)1, and changes in the amide-I (1600–1700 cm)1) region.The frequencies of infrared bands can be modulated by isotope substitution. Growth of the recombinantE. coli ... photophy-sics, and mechanism of pyrimidine dimer repair by DNA photolyase. Photochem Photobiol 57, 895–904.28 Hamm-Alvarez S, Sancar A & Rajagopalan KV (1990)The folate cofactor of Escherichia coli...
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Báo cáo khoa học: Aglycone specificity of Escherichia coli a-xylosidase investigated by transxylosylation ppt
... showed acorrelation between C-1¢ of the xylosyl moiety and H-6 of the fructosyl moiety, and the other showed a corre-lation between C-1¢ of the xylosyl moiety and H-3 of the fructosyl moiety in the ... catalytic domain of each monomeris a triose-phosphate isomerase barrel flanked by twob-sandwich structures. The glycone-binding site isformed by the C-terminal ends of the barrel b strands and their ... overlay-modeled struc-ture of YicI, the regions of 4-OH and 5-OH inthe fructose ring are oriented to Arg446molF and Asp185molF. In the transxylosylation to fructopyranose4-OH and 5-OH of fructopyranose...
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Báo cáo khoa học: Cd2+-induced aggregation of Escherichia coli pyrophosphatase doc
... Physico-Chemical Biology and School of Chemistry, Moscow State University, Moscow, Russia;2Department of Biochemistry, University of Turku, FinlandWe report here that Escherichia coli pyrophosphataseaggregates ... ligands – Asn24 and Asp26 – from two subunitsFig. 1. Time-course of Escherichia coli inorganic pyrophosphatase(PPase) aggregation in the presence of 2 mMcadmium acetate.Fig. 2. Dependence of ... n113Cd2+ and 31P NMR studies. Biochemistry 23, 4947–4955.19. Wang, S.C.K., Hall, D.C. & J osse, J. (1970) C onstitutive i norganicpyrophosphatase of Escherichia coli. 3.Molecularweightandphysical...
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Báo cáo khoa học: Mammalian transglutaminases Identification of substrates as a key to physiological function and physiopathological relevance pot
... 399 of one chain and Lys406 of another fibrin. Successively, the enzyme sta-bilizes the blood clot by cross-linking lysine and gluta-mine of the a and c chains of fibrin molecules [24].Sobel and ... cross-linking of proteins mediated by TG isoforms results in well-defined functions invarious biological processes: cross-linking of fibrin inhaemostasis, of semenogelin in semen coagulation and of involucrin ... in TG isoforms [2]. It consists of an N-terminalb-sandwich, a core (which contains a transamidationsite and a Ca2+-binding site, and has a helices and b sheets in equal amounts), and two...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... 100 lm of (GlcNAc)1–4was analysed at thestart, in the middle and at the end of each series of sam-ples. The resulting average values of the standards (display-ing standard deviations of < ... Materials and methods) . (D) Chromatogram of (GlcNAc)6degradation products generated by LlChi18 after 2 min of incubation with 1 nM of enzyme. The double peaks represent the a- and b-anomers of the ... for by subtracting the value of samples taken at t = 0 min.The determination of the initial products from (GlcNAc)6degradation was performed by incubating 1 nm of LlChi18A with 200 lm of (GlcNAc)6in...
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Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf
... variesbetween 2 and 12 (Table 6). There is one completelyconserved salt bridge between the C-terminal Glu (or Asp) of b-strand B6 (BAX and BCX have Asp) and Arg of theloop between b-strands B7 and A6. ... al.[8],theshapeofthemoleculeresemblesaright hand: two b-sheets and the a-helix form fingers and a palm, a long loop between the B7 and B8 strands forms athumb, and a loop between the B6 and B9 strands forms acord (Fig. ... studies and helps us to under-stand the molecular basis of the thermostability of theseindustrially relevant enzymes.Materials and methods Protein purificationThe catalytic domains of C. thermophilum...
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Tài liệu Báo cáo khoa học: A zymogen form of masquerade-like serine proteinase homologue is cleaved during pro-phenoloxidase activation by Ca2+ in coleopteran and Tenebrio molitor larvae docx
... proteolysis of the55-kDa Tm-mas and PO activity by using two kinds of serine proteinase inhibitors, such as p-NPGB and p-APMSF. As shown in Fig. 7C and D, when HW-55SFig. 6. Localization of the ... concentra-tions were determined by the method of Lowry et al.[16] using BSA as a standard. Molecular cloning of 45-kDa Tm-masA cDNA library from T. molitor larvae was constructed by using a ZAP-cDNA synthesis ... functions of the 45-kDa Tm-masduring pro-PO activation, the PO activity was measured by using HW-55S solution in the presence of b-1,3-glucan and Ca2+. To examine the cleavage of 55-kDa Tm-mas atdifferent...
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