... toRT-mix for synthesis of cDNA. The RT-mix consisted of 6µl of 5X Universal buffer, 3µl of 0.1µl M DDT, 1µl of 10mM dNTP, 1µl of reverse primer (20 pM), 1 of Superscript reverse transcriptase ... Seoul 151-742, KoreaOne step TaqMan reverse transcription polymerase chain reaction (RT-PCR) using TaqMan probe wasdeveloped for detection of Japanese encephalitis virus(JEV). Real-time RT-PCR ... sets for specificity (Fig. 1).Results showed that primer set of 3' NTR had good resultsbut the primer sets of prM and 5' NTR didn’t. Therefore,primers and probe of 3' NTR for the...