Báo cáo khoa học: "Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp Paratuberculosis" pot
... USA).
Antibody response to five recombinant antigens and sero -diagnosis with 35 kDa for
M.
avium
subsp.
paratuberculosis
113
Dialysis of proteins and removal of endotoxins
The recombinant proteins ... by variations in the stage
of infection. In the attempt to increase sensitivity, several
modifications have been tried including using a combination...
... a
175 amino acid p rotein containing only one carbohydrate-
binding d omain (CRD) linked to an N-terminal sequence,
part of which is cleaved during its maturation and secretion
[5]. In humans, ... blotting for HIP/PAP using
polyclonal antibody anti-HIP/PAP and for phosphorylated
serine using polyclonal anti-phosphoserine (Zymed Labor-
atories).
Cell fractionation
HIP/PAP expre...
... shown in Table 1.
Upon pathologic examination with conventional H&E
staining, there was no evidence of metastatic carcinoma
in any of the submitted lymph nodes. Immunostaining
was performed ... dif-
ferentiation, perineural invasion, recurrent lesions, sites
of prior radiotherapy or chronic inflammation, immuno-
compromised states, and genetic disorders including
albinism and...
... lgÆmL
)1
).
Production of HpAKR was initiated by addition of isopropyl
thio-b-d-galactoside to a final concentration of 1 mm, and
this was followed by incubation at room temperature, to
minimize inclusion body formation. ... reduction of aldehydes and ketones to the corre-
sponding alcohol product, and thus serve to remove
potentially cytotoxic and mutagenic aldehydes f...
... 5:89
http://www.implementationscience.com/content/5/1/89
Page 6 of 9
We organize the r eviews of interventions directed to
professionals and the delivery of care using the EPOC
taxonomy of interventions. This taxonomy groups inter-
vent ions into five ... S: Development of a taxonomy of
interventions directed at consumers to promote evidence -based
prescribing and med...
... coordinator),
and instituting at least some minimal form of internal
monitoring that provides corrective feedback to teachers
and administrators regarding implementation.
Competing interests
The ... LST
lessons be a minimum of 50 minutes.
A key aspect of the LST curriculum is variation in instruc-
tors' teaching techniques that includes didactic instruc-
tion, discussion, d...
... stained with Simply Blue Safe Stain
(Invitrogen) according to the manufacturer’s protocol. The
protein concentration was determined using Bio-Rad Pro-
tein Assay based on the method of Bradford ... incubation at 98 °C in a PCR machine for 3 min,
and then kept on ice. Approximately 300 ng of the various
substrates was mixed with 30 ng enzyme in a total volume
of 20 lL con...
... troponin T. ATPase activation and binding to tropo-
nin I and troponin C. J Biol Chem 273, 10594–10601.
31 Pearlstone JR & Smillie LB (1995) Evidence for two-site
binding of troponin I inhibitory ... the
maintenance of structural integrity of the troponin
complex in the relaxed state.
Troponin-tropomyosin based regulation exhibits two
components [32]: inhibition and re...
... inhibitor 2: expression , k inetic analysis of in -
hibition w ith alph a-ch ymotrypsin and wild-type an d m utan t
subtilisin BPN ¢, and protein engineering to investigate inhibitory
speci®city and ... studies of internal dynamics of
serine proteinase protein inhibitors: binding region mobilities of
intact and r eactive-site hydrolized Cucur bita maxima trypsin
inhibitor...
... pres-
ence of trimers in solution. All determinations of native
molecular masses reported here were performed with
excess of Mg
2+
in solution to allow the interactions
involved in hexamer formation. ... UÆmg
)1
protein and recovery levels of 20–
30%. In all cases, the analysis by SDS ⁄ PAGE and
native PAGE of purified preparations showed only
one protein band of 20...