Báo cáo khoa học: " The Antinociceptive and Anti-inflammatory Effect of Ethylacetate Extracts from Bang-Poong (Radix ledebouriellae) on the Freund’s Adjuvant-Induced Arthritis in Rats" pot

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... normalizing against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation ... expression and itsrole in the regulation of other pro -in ammatory genes, suchas TNF and IFN, in ﬁsh, need to be conducted. In conclusion, the IL-10 gene from carp has been isolated and its ... production in mice,chimpanzees, baboons and humans [32–35]. The presence of endogenous IL-10 confers protection from the lethaleffects of endotoxin challenge and reduces the levels of TNF, IFN-c and...

Báo cáo khoa học: Protein aggregation and amyloid ﬁbril formation prediction software from primary sequence: towards controlling the formation of bacterial inclusion bodies pot

... introduction of aggregation-disruptingamino acid substitutions in the aggregation-prone ⁄ amyloidogenic short sequence regions suggests the possibility of ﬁne-tuning and controlling the solu-bility of ... residue of the protein, equal to the probability of backbone–back-bone hydrogen bond formation observed for this type of residue; then, the obtained values are averagedinside the window and the ... for improving the solubil-ity of recombinant proteins and for controlling the for-mation of bacterial inclusion bodies (IBs).Short description of predictionalgorithms and available softwareEach...

Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... dose–response equation of the origin software (OriginLab, Northampton, MA, USA).Afterwards, inhibitor modality was determined by measur-ing the effects of inhibitor concentrations on the enzymaticactivity ... rate of 0.2 mLÆmin)1. The peptidefraction was detected by PDA (TSP UV6000) and directlyintroduced on- line into ESI source. The operating conditionwas optimized with standard solution, and the ... atdesired concentrations. The Km and Vmaxvalues for sub-strates were determined by varying the concentrations of one substrate while keeping the other substrate at satura-tion. In the experiment...

Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of ﬂavonol synthase from Citrus unshiu pptx

... structural integrity of the active FLS, pointmutations were initiated aiming at the substitution of glycine residues by alanine or proline and the exchange of proline by glycine. Glycine residues ... gene, and the essence of most of the conserved amino acids hasbeen further substantiated by site-directed mutagenesis of FHT [7,9] and by documentation of ligand binding in crystalline DAOCS and ... functions in many plants beyond the scope of pigmentation and ultraviolet screening, e.g. in reproduction [1], in the defense against microbial pathogens and insects or in auxin transport [2], and...

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... remarkable as inferred from the NOE data, have little effect on the value of the Æsmiæestimate, and adds conﬁdence to the assumption of isotropic motion adopted by the equation of the relax-ation rate ... [21]. The ﬁt was obtained by linearregression, and only the corresponding J(0)–J(xN)correlation plot is shown in Fig. 4. The localization of the experimental points in Fig. 4along the correlation ... correlation line is directly related to the dis-tribution of the energy between the overall tumbling and the internal mobility, and is indicative of the degree of internal restraint of each15N–1H...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... with reaction mixtures containing only the indicated (d)NTPs in addition to the DNA template and the protein,whereas lane 0 contains a control reaction without protein.Analysis of the pIT3 prim–pol ... both the R186 residue in the Rep245 domain and K300, its counterpart in Pfu-primase, werecontained in a loop that is plausibly involved in DNArecognition and binding and is positioned left of the ... and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu-bating the protein at 60, 70 and 80 °C for 30 min and thenrecording the CD spectra of the incubated samples at indi-cated...

Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot

... [13].Except for the detailed studies on crustacyanin, the lobster carapace carotenoprotein, there is little infor-mation on the structure and stability of this interestinggroup of proteins, and there ... giving the best fit to the scattering intensity. The resulting dummy atom model represents the shape of the scattering particle. To increase the reliability of the results, the final model for the ... shape and dimensions, and differs from data on other inver-tebrate carotenoproteins such as the lobster crusta-cyanin (a cylinder of 238 · 95 A˚) [21] and the starfishlinckiacyanin (a spring-like...

Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... numbers on the left of the figure indicate the positions of the deduced amino acid sequence. N-terminal segment determined by protein sequencing is underlined. (B) Comparison of the amino acidsequence ... proteins including isoforms ⁄ degradedprotein products and around 65 of these are IgE-bindingproteins [19]. Only a few of these proteins have beenpurified and characterized so far. In the present ... sequence and sequence alignment of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numbers on the right of the figure indicate the positions of the nucleotide sequence. The...

Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... & Zhao, S. (2000) Cloning and mapping of humanPKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhibitor family, includingPKIA. Biochem. J. ... amide I¢component bands, determined by band ﬁtting of the absorbance spectrum of Fig. 4A, are shown in Fig. 6 and Table 2. From Table 2, the sum of individual amide I¢intensity and the intensity ... resolution of 0.2 nm and accumulated for four scans. Protein concentra-tion was 0.071 mgÆmL)1 in H2O.ResultsCloning of the gene coding for human PKIb The cDNA clone encoding PKIb gene contains...

Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx

... ubiquitin-like (UbL) domain, the XPC-bindingdomain, and two ubiquitin-associated domains (UBA1 and UBA2) [9,10]. The UbL domain has a high bind-ing affinity for polyubiquitin binding site 2 of the human ... because of secondary effects from the divergent positioning of the flexible N-terminal region (275–283), the boundary and size of HT1 were variable; in contrast, this vari-ability of the HT1 region ... beanother advantage of adapting to the larger bindingcounter part. The difference in flexibility of the N-terminal regions of XPCB–hHR23B and XPCB–hHR23A may be oneway to explain why these domains,...

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