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Báo cáo khoa học: "Comparative study of endocrine cells in the principal pancreatic islets of two teleosts, Silurus asotus (Siluridae) and Siniperca scherzeri (Centropomidae)" potx

Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

... which was indeed predicted. The other maingroup, obtained 97% of the time in 20 740 exploredtrees, contained the DIDO PHD sequences clusteredwith the PHF and the CGBP proteins. As seen in the tree, ... distribution (Fig. 1), in which the SPP1 representatives are at the basal branch of CGBP and DIO. The GCBP proteins contain a PHD domain, fol-lowed by a DNA-binding domain (the zf-CXXC) and the newly described ... modifications of chromatin [9]. Little isknown, however, of the functional role of the remain-ing subclass members. In this study, the yeast protein SPP1 (alias cps40), amember of the COMPASS...
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Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf

Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf

... unfolding occurs. The detailed dissociation/unfold-ing mechanism of the protein remains to be determined. In this study, we examined the Mr of the protein afterequilibration in various denaturing ... further increased. The unfolding curveusing the ANS fluorescence probe suggested a multistateprocess, consistent with the results obtained by proteinintrinsic fluorescence and CD. The binding ... (velocity of sedimentation dividedby the acceleration of the force field) of 9.14 s (Fig. 3).Incubation of the protein in 0.2Mor 0.4MGdnHClresulted in a decrease in the s-value to 8.87 s and...
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Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx

Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx

... chain is brefeldin A-insensitive and isinhibited by the proteasome inhibitor clasto-lactacystinb-lactone, resulting in the accumulation of ricinA chains. These stabilized ricin A chains are partlydeglycosylated ... (cyclophilin) survey the status of the proline residues and rearrange themfrom the cis to the trans form to ensure proper folding of the nascent polypeptide chains. Twenty-nine genesencoding cyclophilin ... transduced from the ER to the nucleus, and thisfinding opened the door to investigation of the details of UPR signaling events. In comparison with that of yeast, the UPR of mam-malian cells is a...
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Báo cáo khoa học: A di-leucine sorting signal in ZIP1 (SLC39A1) mediates endocytosis of the protein doc

Báo cáo khoa học: A di-leucine sorting signal in ZIP1 (SLC39A1) mediates endocytosis of the protein doc

... the surface of these cells were internalized after 1 h of incubation(Fig. 5H), indicating that the endocytic machinery in these mutant ZIP1-Myc expressing cells was intact.Taken together, these ... contrast, in the presence of chloroquine, the total ZIP1-Myc accumula-tion in the CHO cells expressing the wt ZIP1-Myc pro-tein was increased to the level that was detected in the cells expressing the ... membrane into the cytoplasmic compartment by the di-leucine signal of ZIP1 and substitutions of the di-alanine residuesfor the di-leucine residues in the ZIP1 sorting signalinhibited the internalization...
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Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterfly, Papilio xuthus docx

Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterfly, Papilio xuthus docx

... staining of the gel indicates that the fluorescing protein is one of the major components of soluble proteins in the crude extract. The surface of the fluorescing protein carries negative charge in ... kind of retinol-binding protein possibly localized in the Papilio retina, and functions in the visual cycle and/ orcolor vision. In this study, we therefore isolated a soluble retinol-binding ... cellular retinal-binding protein; CRBP,cellular retinol-binding protein; IRBP, interphotoreceptorretinoid-binding protein; RBP, retinol-binding protein.Note: The nucleotide sequence reported in this...
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Báo cáo khoa học:

Báo cáo khoa học: "Feasibility Study for Ellipsis Resolution in Dialogues by Machine-Learning Technique" docx

... sufficient, and sometimes more. In this section, we investigate decision trees and discuss them in detail. 5.1 Tree Shape The relation between the number of training samples and the number of nodes ... Japanese thesaurus. 4 Discussion In this section we discuss the feasibility of the el- lipsis resolver via a decision tree in detail from three points of view: the amount of training data, the ... the easiest of the four cases for characterizing the individuality among the ellipsis types. Table 6 shows node depth and the maximum width in the decision trees we have built. By studying...
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Báo cáo khoa học: Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases ppt

Báo cáo khoa học: Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases ppt

... mutant) and shows the PCP on the acceptor site of the C-domain. The dis-tance of the S1003A residue to the active-site histidine of the C-domain is 16 A˚, making it within the reach of the missing ... consisted of the first two functional domains, namely the phenylalanine-specific A domain and the PCP domain. The terminalE domain was excised (the exact amino acid composi-tion of the investigated ... by decreasing the rate of cleavagebetween the two subdomains of the A domain. Thesefindings were later explained with the structural modelthat the smaller subdomain AC of the A domainrotates...
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Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot

Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot

... clarify the following issues through futurelong-term in vivo studies: (a) evaluation of silencing of the promoter, and the resultant level of coexpression of SUMF1 and GALNS; and (b) confirmation of ... design the experiments and drafted the manuscript. A. M. Montan˜o conceived and designed the experiments, and helped in analysis of the results and drafting of the manuscript. S. Tomatsu and L. ... [6].Gene therapy is a promising alternative approach, and there have been a number of clinical and experi-mental studies. The success of a gene therapy protocoldepends on the selection of the candidate...
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Tài liệu Báo cáo khoa học: A genetic screen identifies mutations in the yeastWAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation docx

Tài liệu Báo cáo khoa học: A genetic screen identifies mutations in the yeastWAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation docx

... DNA-binding properties can be different in vitro and in vivo and may involve sequences in the regula-tory domains distinct from the zinc finger or evenadditional factors [34]. Therefore, the ... sorbic,benzoic and propionic acid [7] and fail to adapt to suchstress conditions in the absence of Pdr12p. Moreover,recent data indicate the involvement of Pdr12p in the export of by-products of amino-acid ... unaf-fected, we determined whether War1-42p still binds to the WARE in the PDR12 promoter. To clarify this,we performed in vivo footprinting experiments in cells expressing War1-42p. The strains YPH499...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "A HYBRID APPROACH TO REPRESENTATION IN THE JANUS NATURAL LANGUAGE PROCESSOR" pot

... defining the set of items that have ever been the actor in a flight. Their definition is solely within NIKL using the QUA link [14], which is exactly the set of fillers of a slot. While having ... using the defini. tJons of those vocabulary items themselves in the dictionary. In this attempt, we encountered the follow- ing difficulties: • Definitions of the base vocabulary often in- ... vessel and speed is ac- quired. In acquiring the definitions of vessel and speed, the system should infer interpretations for phrases such as the speed of a vessel, the vessel's speed, and...
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