... after the last CATG site before the polyA signal in the PCR product, if possible, before further analysis. If the presence of the tagis confirmed, the additional nucleotide sequence obtained, ... PCRtemplate. For PCR, the tag-specific primer was used as the sense primer, and consisted of the 14-base tagnucleotides and 5¢ inosine nucleotides to increase the annealing temperature of the primers ... (A) In this process, first-strand cDNA synthesized by oligo(dT) is used for PCR. In the first cycle, the templatewith the SAGE tag binding site is annealed to the sense primer and extended to the...